Method for constructing human peripheral blood immune cell bank

Abstract

The invention discloses a method for constructing a human peripheral blood immune cell bank. The method comprises the following steps of: collecting human peripheral blood, separating autologous plasma, separating a peripheral blood mononuclear cell, separating a mononuclear cell by the peripheral blood mononuclear cell and freezing, separating a T lymphocyte by the peripheral blood mononuclear cell and freezing, separating a B lymphocyte by the peripheral blood mononuclear cell and freezing, separating an NK cell by the peripheral blood mononuclear cell and freezing, and encoding and puttingin storage. According to the invention, immune cells of health or young people are separated and are respectively independently frozen, and relative numbers are stored and put into storage, so that the stored human immune cells have the characteristics of high activity, high purity and convenience for use, and fetal calf serum can be replaced by the human autologous plasma, so that introduction of a foreign protein can be avoided. Meanwhile, the method, provided by the invention, has the advantages of low cost, low requirements on laboratory conditions, and wide application.

Description

Technical field [0001] The invention relates to a method for constructing a cell bank, in particular to a method for constructing a human peripheral blood immune cell bank. Background technique [0002] Immune cells refer to all cells related to immune response, mainly including T cells, B cells, killer cells, natural killer cells, monocytes, etc. In recent years, tumor and virus immunotherapy technology has been slowly applied to the clinic. Such as tumor vaccines and adoptive immunotherapy, these methods mainly use autologous immune cells to be stimulated, cultured, expanded and then infused in vitro to improve the immunity of patients. At present, such cells mainly include DC, CIK, NK, and DC-CIK. Wait. [0003] These immune cell treatments are mainly taken from the patient’s own peripheral blood, which is achieved by obtaining peripheral blood mononuclear cells and then undergoing a series of operations. However, the general population receiving immune cell treatment is mainl...

AI Technical Summary

Problems solved by technology

However, the composition of human peripheral blood mononuclear cells is complex, including both immune cells and non-immune cells. Even immune cells are a combination of various types of cells. These cells have different requirements for cryopreservation conditions. Cryopreservation, because the cryopreservation conditions are not optimal, the survival rate of cryopreserved mononuclear cells is very unsatisfactory when resuscitated, and cannot meet clinical requirements

At the same time, the current cryopreservation solution for cryopreserving cells generally contains fetal bovine serum, which contains a variety of proteins, which are foreign proteins compared with the human body, and there are hidden dangers in application

It has also been reported to use serum-free cryopreservation solution, but the serum-free cryopreservation solution is expensive and has complex components, making it difficult to promote clinically.

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