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Method for purifying beta-sitosterol from cornel kernels

A technology of cornus kernel and sitosterol, which is applied in the field of separation and purification of biologically active substance phytosterol, can solve the problems of low purity of beta-sitosterol, difficult to achieve and high cost, and achieves good crystal shape, high separation speed and low cost. Effect

Inactive Publication Date: 2012-10-31
刘拴柱
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing technologies for separation and purification of β-sitosterol in China have problems such as low yield, high cost, and low purity of β-sitosterol (only about 60%, and it is difficult to reach more than 90%).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for purifying β-sitosterol from cornus officinale, comprising the following steps:

[0019] 1) Crush the fruit core of dogwood into 15 mesh particles;

[0020] 2) Soak the crushed granules in 5 times the weight of 95% edible ethanol for 24 hours, filter, and squeeze out the oil;

[0021] 3) Combine the filtrate and squeezed liquid, concentrate under reduced pressure to remove the ethanol in the combined liquid, then mix the oil after removing ethanol with NaOH solution with a concentration of 25% (W / W) according to M / V=1:1, at 75 degrees Saponification under reflux for 3 hours;

[0022] 4) cooling the reaction solution prepared in step 3), and lowering the temperature to 0°C to precipitate the residue, filter to remove the saponification product, and concentrate the filtrate to 1 / 10 of the original volume under reduced pressure at 65°C;

[0023] 5) Add an equal volume of ethyl acetate to the filtrate to extract the filtrate 2 to 3 times, combine the extracts,...

Embodiment 2

[0030] A method for purifying β-sitosterol from cornus officinale, comprising the following steps:

[0031] 1) Crush the fruit core of dogwood into 20 mesh particles;

[0032] 2) Soak the crushed granules in 5 times the weight of 95% edible ethanol for 24 hours, filter, and squeeze out the oil;

[0033] 3) Combine the filtrate and squeezed liquid, concentrate under reduced pressure to remove the ethanol in the combined liquid, then mix the oil after removing ethanol with NaOH solution with a concentration of 25% (W / W) according to M / V=1:1, at 75 degrees Saponification under reflux for 3 hours;

[0034] 4) cooling the reaction solution prepared in step 3), and cooling it down to 0°C to precipitate the residue, filter to remove the saponification product, and concentrate the filtrate to 1 / 10 of the original volume under reduced pressure at 65°C;

[0035] 5) Add an equal volume of ethyl acetate to the filtrate to extract the filtrate 2 to 3 times, combine the extracts, adjust t...

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Abstract

The invention relates to a method for separating and purifying a biological active substance phytosterin, particularly a method for quickly and massively extracting high-purity beta-sitosterol from cornel kernels. The high-purity beta-sitosterol is quickly and massively extracted from the cornel kernels by saponification, extraction, fractional crystallization and other techniques. The invention has the advantages of high separation speed, low separation cost, high yield, abundant and accessible raw material, and the like. By fully utilizing the residual kernel waste after processing the traditional Chinese medicine cornel, the method provided by the invention has the advantages of low cost, high yield, high product purity (the purity is higher than 98%, and the melting point is 140-141 DEG C) and favorable crystal shape (white acicular crystal); and the beta-sitosterol is suitable to be used as a sitosterol standard substance and medicinal raw material.

Description

Technical field: [0001] The invention relates to a method for separating and purifying bioactive substance phytosterol, more specifically, relates to a method for rapidly and batch-extracting high-purity β-sitosterol from cornus officinalis. Background technique [0002] β-sitosterol is an important raw material for the production of steroidal drugs and vitamin D3, especially the key intermediate of the anti-SARS co-drug "glucocorticoid"; it has obvious effects on cancer, such as the treatment of cervical cancer and skin cancer Curative effect; it has the effect of inhibiting the body's absorption of cholesterol, promoting the degradation and metabolism of cholesterol, and inhibiting the biochemical synthesis of cholesterol; and β-sitosterol can be used to prevent and treat coronary heart disease and atherosclerotic heart disease; It has strong anti-inflammatory and antipyretic effects, similar to hydrocortisone, phenylbutazone and aspirin, and has no side effects of such dr...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 刘拴柱
Owner 刘拴柱
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