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Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array

A bipolar electrode, luminescence detection technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve problems such as detection of microfluidic droplet arrays that have not yet been seen, and achieve fast electrochemical reaction, Detect effects with low background and low volume

Inactive Publication Date: 2012-10-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, this new detection mode has been widely used in the sensing, separation and enrichment of targets in microfluidic systems, and there is no report on the detection of microfluidic droplet arrays.

Method used

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  • Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array
  • Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array
  • Bipolar electrode electrochemiluminescent detection method for microfluidic droplet array

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preparation example Construction

[0021] The preparation of the microfluidic chip can adopt the standard soft lithography technology to manufacture the indium oxide conductive glass substrate 3 provided with the driving electrode 1 and the bipolar electrode 2, and adopt the molding method to process the micro-droplet micro-reservoir 5 of the reagent. and the polydimethylsiloxane cover sheet 4 of the sample micro-droplet micro-reservoir array 6, and seal the two to obtain the chip used.

[0022] The detection method of a bipolar electrode electrochemiluminescence detection microfluidic droplet array device, respectively adding reagent microdroplets and sample microfluidics to the reagent microdroplet microreservoir 5 and the sample microdroplet microreservoir array 6 A constant potential of 1.50–5.25 V is applied to the driving electrode 1 to promote redox reactions at both ends of the bipolar electrode 2, and the microfluidic droplet array can be detected by observing the electrochemiluminescent signal emitted ...

Embodiment 1

[0024] The detection method of the bipolar electrode electrochemiluminescence detection microfluidic droplet array device is as follows: respectively add 5 μ L containing 1 × 10 -3 M terpyridine ruthenium chloride and 2×10 -2 M dibutylaminoethanol sample micro-droplets and 80 μL containing 0, 1 x 10 -5 or 5×10 -3 M potassium ferricyanide reagent micro-droplet solution, apply a constant potential of 5.25 V, use a CCD camera to detect the electrochemiluminescence signal at the anode end of the bipolar electrode, and obtain the following: image 3 Luminescence image results shown.

Embodiment 2

[0026]The detection method of the bipolar electrode electrochemiluminescence detection microfluidic droplet array device is as follows: respectively add 20 μL containing 1×10 -5 or 2×10 -4 M terpyridine ruthenium chloride and 2×10 -2 The sample droplet of M dibutylaminoethanol and 150 μL of the reagent droplet solution containing 0 M potassium ferricyanide were applied with a constant potential of 4.75 V, and the electrochemiluminescence signal at the anode end of the bipolar electrode was detected by a CCD camera. get as Figure 4 Luminescence image results shown.

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Abstract

The invention discloses a bipolar electrode electrochemiluminescent detection device for a microfluidic droplet array and a detection method thereof. According to the invention, a bipolar electrode and a drive electrode are processed on an indium tin oxide conductive glass substrate, a sample micro droplet micro liquid storage tank array and a reagent micro droplet micro liquid storage tank are processed on a polydimethylsiloxane cover plate, so a polydimethylsiloxane-conductive glass microfluidic chip is prepared by sealing the substrate and the cover plate; sample micro droplets and reagent micro droplets with a micro upgrading volume are respectively added into micro liquid storage tanks at the two ends of the bipolar electrode, and a constant potential is applied onto the drive electrode so as to allow the two ends of the bipolar electrode to undergo a redox reaction; and detection of the microfluidic droplet array is realized by observing an electrochemiluminescent signal emitted by an anode terminal of the bipolar electrode. The method provided in the invention has the advantages of simple operation, a small consumption amount of a sample and a reagent, high sensitivity, a short analysis time and capacity of realizing multi-point detection at the same time.

Description

technical field [0001] The invention belongs to a microfluidic droplet array detection method, in particular to a method for detecting a microfluidic droplet array based on bipolar electrode electrochemiluminescence. Background technique [0002] The research on microfluidic droplet system is an important development direction of microfluidics, which has attracted extensive attention of experts and scholars at home and abroad. This is mainly due to the potential of microfluidic droplet systems as microreactors for high-throughput analysis. At present, droplet microfluidic systems have been widely used in chemical synthesis, protein crystallization, single-molecule detection, and single-cell analysis. [0003] Electrochemistry is a commonly used analytical detection technique. Since the electrode system can be easily miniaturized without reducing its detection sensitivity, electrochemical technology is considered to be a detection technology with the greatest potential to m...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 苏彬方群吴锁柱
Owner ZHEJIANG UNIV
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