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Method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking with hot-water ultrasonic extracting

A technology of ultrasonic extraction and bee pollen, applied in the chemical field, can solve the problems of complicated operation steps and the like

Inactive Publication Date: 2012-10-24
TONGLU XINGYUAN HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The patent number is 2011103908202 and the name is the preparation method of the active ingredient polysaccharide in the schisandra medicinal residue, and the patent number is 2011103653237. In the two inventions, the name is a separation and purification method of the dendrobium polysaccharide, both adopt the Sevage deproteinization method, and the disadvantage is that both require Repeat the deproteinization for about 5 times with the Sevage deproteinization method to obtain polysaccharides with high purity, and the operation steps are cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: This embodiment is made up of the following steps:

[0040]1. Firstly, the bee pollen is dried, impurity-removed and screened, then mechanically crushed and sieved to form bee pollen powder;

[0041] 2. Add 0.5% mass of cellulase to the bee flower powder, then add 9 times the mass of water, and mix to form a cellulase bee flower powder mixture;

[0042] 3. Heating the cellulase bee flower powder mixture, the temperature is controlled at 42 degrees, and the enzyme decomposition reaction time is 90 minutes; the bee pollen aqueous solution is formed into a paste after the wall is broken;

[0043] 4. Heat the pasty aqueous solution of bee pollen after breaking the wall. The temperature is controlled at 82 degrees, the ultrasonic power is 400W, and the leaching is carried out twice for 2.5 hours each time. The polysaccharide in the pasty aqueous solution of bee pollen is completely decomposed in the aqueous solution. ;

[0044] 5. Add organic solvent pure edi...

Embodiment 2

[0051] Embodiment 2: This embodiment is made up of the following steps:

[0052] 1. Firstly, the bee pollen is dried, impurity-removed and screened, then mechanically crushed and sieved to form bee pollen powder;

[0053] 2. Add 0.1% mass of cellulase to the bee flower powder, then add 6 times the mass of water, and mix to form a mixture of cellulase and bee flower powder;

[0054] 3. Heating the cellulase bee flower powder mixture, the temperature is controlled at 42 degrees, and the enzyme decomposition reaction time is 90 minutes; the bee pollen aqueous solution is formed into a paste after the wall is broken;

[0055] 4. Heat the pasty aqueous solution of bee pollen after breaking the wall. The temperature is controlled at 82 degrees, the ultrasonic power is 400W, and the leaching is carried out twice for 2.5 hours each time. The polysaccharide in the pasty aqueous solution of bee pollen is completely decomposed in the aqueous solution. ;

[0056] 5. Add 30% mass of orga...

Embodiment 3

[0063] Embodiment 3: This embodiment is made up of the following steps:

[0064] 1. Firstly, the bee pollen is dried, impurity-removed and screened, then mechanically crushed and sieved to form bee pollen powder;

[0065] 2. Add 1% mass of cellulase to the bee flower powder, and then add 8 times the mass of water to form a mixture of cellulase and bee flower powder;

[0066] 3. Heating the cellulase bee flower powder mixture, the temperature is controlled at 42 degrees, and the enzyme decomposition reaction time is 90 minutes; the bee pollen aqueous solution is formed into a paste after the wall is broken;

[0067] 4. Heat the pasty aqueous solution of bee pollen after breaking the wall. The temperature is controlled at 82 degrees, the ultrasonic power is 400W, and the leaching is carried out twice for 2.5 hours each time. The polysaccharide in the pasty aqueous solution of bee pollen is completely decomposed in the aqueous solution. ;

[0068] 5. Add 40% mass of organic sol...

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Abstract

The invention discloses a method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking and hot-water ultrasonic extracting. The method comprises the following steps: drying, purifying, grinding and screening bee pollen; carrying out enzymolytic wall-breaking; carrying out heating and ultrasonic extracting; dehydrating; deproteinizing with perchloric acid; carrying out (diethylamino) ethyl column chromatography and elution; separating acid polysaccharide precipitation and neutral polysaccharide precipitation with cetyl trimethyl ammonium bromide (CTAB); and dissolving the polysaccharide precipitations, then dehydrating, vacuum-freezing, and drying to obtain the high-purity impurity-free bee pollen polysaccharide. By the method, the polysaccharide extraction ratio is improved by 66.03%, and the polysaccharide yield reaches 18.54mg / g which is improved by 2.02 times compared with that of the extraction method of the prior art; and the obtained bee pollen polysaccharide liquid is negative relatively to the ninhydrin reaction, no protein residual is left, and the obtained bee pollen polysaccharide has no impurities and high purity.

Description

technical field [0001] The invention relates to a method of bee pollen polysaccharide enzymolysis and wall breaking combined with hot water ultrasonic extraction, which belongs to the technical field of chemical industry. Background technique [0002] Bee pollen contains a certain amount of protein, free amino acids, various active enzymes, various vitamins, nucleic acids, minerals, carotene, auxin, phospholipids, sugars and other nutrients. Green food is known as the concentrate of nutrients. Modern research has proved that bee pollen can extract high-level T lymphocytes in the body (T lymphocytes are derived from the original T cells of embryonic liver or bone marrow, differentiate and develop in the thymus microenvironment, and settle in the lymphoid tissue in the peripheral blood after maturity; T cells can Mediate cellular immune response, and assist the body to produce humoral immunity against T cell-dependent antigens.) and macrophages, have the ability to increase s...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08B37/06C08B30/04
Inventor 袁文明
Owner TONGLU XINGYUAN HEALTH PROD
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