Thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and detection method thereof

Abstract

The invention discloses a thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit and a detection method thereof. The thyroperoxidase antibody homogeneous-phase luminescent immunoassay kit comprises a homogeneous-phase luminescent 96-pore determination plate, a thyroperoxidase antibody standard product, a streptavidin coated donor microsphere solution and a biotin labelled thyroperoxidase antigen, as well as a thyroperoxidase antigen coated receptor microsphere solution. The thyroperoxidase antigen in a sample is competitive with the thyroperoxidase antigen on the receptor microspheres to combine with the biotin labelled thyroperoxidase antigen. The detection method comprises the steps of measuring the luminescence signals of known standard products with different contents, drawing a standard curve and obtaining a mathematical function, and obtaining the content of unknown sample by the mathematical function. The detection method integrates the advantages of both enzyme immunoassay and chemiluminescence immunoassay, and accurately determines the level of the thyroperoxidase antibody in serum, is applied for screening, diagnosing and curative effect evaluation on high-risk groups with autoimmune thyroid disease.

Description

technical field [0001] The invention relates to a light excitation coupling immunoassay technology, in particular to a homogeneous luminescent immunoassay kit for thyroid peroxidase antibody and a detection method thereof. Background technique [0002] Thyroid peroxidase (TPO) is a membrane-bound glycoprotein containing a heme prosthetic group. The whole molecule is divided into an inner region, a transmembrane region and an outer region. active. As an important enzyme in the synthesis of thyroid hormone, TPO can catalyze the activation of iodine, the iodination of tyrosine residues on thyroglobulin and the coupling of iodinated tyrosine residues, and finally generate thyroxine (T 3 , T 4 ). When thyroid disease occurs and the follicular cell structure is destroyed, TPO leaks from the edge of the thyroid follicular cell cavity, that is, the top of the thyroid cells, to the peripheral blood, stimulating the body's immune system to produce TPO antibodies (anti-TPO antibodie...

AI Technical Summary

Problems solved by technology

[0009] Second: Inherent defects brought about by the indirect method response mode

There are inherent defects in this indirect detection mode, which are as follows: ① Since the enzyme-labeled antibody is only for IgG, it cannot detect other types of antibodies (such as IgM or IgA); Heterosexual antibodies will cause some cross-reactions, and the serum to be tested needs to be diluted to reduce non-specific binding, and serum dilution will bring certain errors

Images