Application of N-acetylcysteine in in-vitro amplification of absolute quantity of central memory T cells

Abstract

The invention discloses an application of N-acetylcysteine in the in-vitro amplification of the absolute quantity of central memory T cells, and provides an optimal concentration for N-acetylcysteine to promote in-vitro amplification. The method is characterized in high safety, low cost, and the like. The method provides good technical support for general development of adoptive immunotherapy.

Description

technical field [0001] The present invention relates to the application of N-acetylcysteine ​​to the absolute number of central memory T cells in vitro expansion. Background technique [0002] N-acetylcysteine ​​(N-Acetylcysteine, NAC for short) is the precursor of intracellular reduced glutathione (GSH), NAC molecular formula: C 5 h 9 NO 3 S, relative molecular mass: 163·20. NAC has been used as a mucolytic agent for many years in the treatment of various respiratory diseases. In recent years, it has been found that NAC has a protective effect on liver cells and is used to treat liver cell damage caused by various reasons. Among them, NAC has been approved by the US FDA for the treatment of acute liver failure caused by acetaminophen. Recent studies have shown that NAC has a variety of pharmacological effects. It can directly scavenge free radicals, increase the body's ability to resist oxidative stress, and reduce the production of inflammatory cytokines, chemokines an...

AI Technical Summary

Problems solved by technology

However, such tumor hyperresponsive cells need to be expanded to clinically required 10 9 -10 11 The number of treatment doses and the duration of the process are relatively long, and time is also a factor that needs to be considered more for cancer patients

Moreover, after a long time of in vitro culture, the cells are prone to aging and their vitality decreases. Most of them are terminally differentiated cells, which have better effects in vitro, but the survival time in vivo is shorter, and the therapeutic effect is affected.

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