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Method for preparing and extracting carotenoid from microbial thalli

A technology of microbial bacteria and carotene, which is applied in the field of bioengineering and bioseparation engineering, can solve the problems of high energy consumption and other costs, large amount of organic solvent consumption, and low yield of single extraction, so as to reduce energy consumption and other costs , quality controllable, short time effect

Active Publication Date: 2012-10-17
HUAZHONG UNIV OF SCI & TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These extraction methods all require dehydration and drying and bacterial crushing processes, the process is long, the single extraction yield is low and takes a long time, the amount of organic solvent is large, the energy consumption and other costs are high, and the industrial application is limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] After the fermentation of B. trispora, the sediment was left to stand, and the supernatant was discarded to obtain wet cells. The wet bacteria were introduced into the blasting tank through the logistics pipeline, heated to a saturated steam pressure of 0.1MPa with 0.3MPa steam and blasted, and centrifuged to obtain a filter cake of bacteria. , filtered to obtain a clear extract, concentrated in vacuum at 50° C. and vacuum degree of 0.01MPa, and recovered acetone to obtain carotenoid oil containing β-carotene, γ-carotene and lycopene. The recovery rate of β-carotene reached 90%, the recovery rate of γ-carotene reached 93%, and the recovery rate of lycopene reached 92%.

Embodiment 2

[0022] After the fermentation of B. trispora, the wet cells were obtained by centrifugation. The wet bacterial cells were introduced into the blasting tank through the logistics pipeline, heated to a saturated vapor pressure of 0.2 MPa with 0.1 MPa steam for steam explosion, and vacuum filtered to obtain a bacterial cell filter cake. 30% by volume), stirring and leaching at 35°C for 50 minutes, filtering to obtain a clarified extract, vacuum concentration at 40°C and a vacuum of 0.05MPa, and recovering the organic solvent to obtain β-carotene, γ-carrot containing carotenoid oil of lycopene and lycopene. The recovery rate of β-carotene was 93%, the recovery rate of γ-carotene was 92%, and the recovery rate of lycopene was 95%.

Embodiment 3

[0024] After the fermentation of B. trispora, the sediment was left to stand, and the supernatant was discarded to obtain wet cells. The wet bacterial cells are introduced into the blasting tank through the logistics pipeline, heated to a saturated vapor pressure of 0.15 MPa with 0.6 MPa steam, and then blasted by the plate and frame filtration to obtain a bacterial filter cake. 50% by volume), stirring and leaching at 40°C for 45 minutes, filtering to obtain a clear extract, vacuum concentration at 60°C and a vacuum of 0.08MPa, and recovering ethanol and acetone to obtain β-carotene, γ-carrot containing β-carotene and γ-carrot. carotenoid oil of lycopene and lycopene. The recovery rate of β-carotene was 93%, the recovery rate of γ-carotene was 93%, and the recovery rate of lycopene was 94%.

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Abstract

The invention which belongs to bioengineering and bioseparation engineering fields relates to a method for preparing and extracting carotenoid from microbial thalli. The method comprises the following steps: fermenting the microbial thalli, separating the resulting fermentation solution and the thalli to obtain wet thalli, guiding the wet thalli to an explosion tank through a material flow pipeline, and carrying out high-pressure explosion wall breaking; dehydrating the wet thalli with disrupted cells to obtain a filter cake formed by thallium fragments; adding 10-25L of an organic solvent to each 1kg of the filter cake, and carrying out stirring extraction at 30-55DEG C for 20-50min; filtering to obtain an organic solvent extraction containing carotenoid grease; and carrying out vacuum concentration on the extraction at 40-60DEG C, and recovering the organic solvent to obtain the carotenoid grease. The extraction yield one time reaches above 90%. The method has the advantages of omission of dehydration drying and thallium crushing operations in traditional technologies, short process flow, high extraction yield and short time each time, reduced organic solvent application amount, substantially reduced energy consumption and other costs, simple whole process, and controllable quality, and is suitable for industrialized production applications.

Description

technical field [0001] The invention belongs to the fields of biological engineering and biological separation engineering, and particularly relates to a method for extracting and preparing carotenoids from microbial cells. The method is especially suitable for the preparation of carotenoids in large quantities from B. trispora and Phaffia rhodozyma. Background technique [0002] Beta-carotene, gamma-carotene, lycopene, and astaxanthin are the four major carotenoids in microorganisms. Beta-carotene, lycopene, and astaxanthin are lipophilic compounds with very high market value. It is a natural pigment in the food industry, an anti-aging antioxidant in the cosmetic industry, and an anti-cancer drug in the pharmaceutical industry. The demand for these carotenoids in the food, cosmetics, and pharmaceutical industries is also increasing, and the market prospects are good. At present, lycopene is mainly extracted from tomato, and β-carotene is mainly extracted from salina sali...

Claims

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Application Information

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IPC IPC(8): C09B61/00C07C403/24C07C403/02C07C11/21C07C7/00
Inventor 余龙江王红波鲁明波何谧何峰
Owner HUAZHONG UNIV OF SCI & TECH
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