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Preparation technology of stilbene glucoside in polygonum multiflorum

A technology of stilbene glycosides and Polygonum multiflorum, which is applied in the preparation of sugar derivatives, medical preparations containing active ingredients, sugar derivatives, etc., can solve the problems of unsuitable for industrial production, small scale, complicated process, etc.

Inactive Publication Date: 2012-10-17
BEIJING SL PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction process is relatively advanced, and the content and purity of stilbene glycosides in the final product are relatively high, but the process is complicated and the scale is relatively small, so it is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 (preparation of stilbene glycoside extract)

[0020] Take Polygonum multiflorum medicinal material 100kg, add 8 times the amount of 50% ethanol, heat and reflux extraction 3 times, 2 hours each time, combine the extracts, concentrate to 1 / 20 of the total volume, let the concentrate stand for 8 hours, filter, add the filtrate 1.5 times the amount of ethyl acetate was extracted three times, the extracts were combined, the ethyl acetate was recovered and concentrated to a thick paste, dried under reduced pressure (75°C), and crushed into fine powder to obtain crude stilbene. The crude product was redissolved in water, dried under reduced pressure (75°C), and crushed into a fine powder to obtain the stilbene extract. The content of stilbene glycosides in the extract is 58.4%, and the content of anthraquinones is 4.1%.

Embodiment 2

[0021] Embodiment 2 (preparation of stilbene glycoside extract)

[0022] Take 100kg of Radix Polygoni Multiflori, add 8 times the amount of 60% ethanol, heat and extract 3 times, 2 hours each time, combine the extract, concentrate the extract to 1 / 10 of the extract, let it stand for 8 hours, filter, add 1.5 to the filtrate Extract with twice the amount of ethyl acetate for 3 times, combine the extracts, recover the ethyl acetate and concentrate to a thick paste, dry under reduced pressure (75°C), grind into fine powder, and obtain the crude stilbene glycoside. The crude product was redissolved in water, dried under reduced pressure (75°C), and crushed into a fine powder to obtain the stilbene extract. The content of stilbene glycosides in the extract is 60.6%, and the content of anthraquinones is 3.8%.

Embodiment 3

[0023] Embodiment 3 (preparation of stilbene glucoside fine product)

[0024]Take 25 g of the crude product of stilbene glycosides, dry-load it to a silica gel column (500 g of silica gel, wet-packed, diameter-to-height ratio is 1:10) and elute with chloroform:methanol=5:1, collect the eluate, and The results of layer detection were combined with similar fractions, concentrated and dried under reduced pressure. Dissolve the dried sample in an appropriate amount of 20% methanol aqueous solution, load the sample to the treated Sephadex LH-20 column, elute with 20% methanol aqueous solution, monitor the purity of stilbene glycosides in the fraction by thin-layer chromatography, and collect similar groups points, concentrated to a thick paste, and dried to obtain the refined stilbene glycoside with a purity of 97.3%.

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Abstract

The invention discloses a preparation technology of a stilbene glucoside extract product in polygonum multiflorum and a production technology of a stilbene glucoside fine product by using a stilbene glucoside crude product as a raw material. The preparation technology of the stilbene glucoside extract product comprises the following steps of: extracting the medicinal material polygonum multiflorum by a dilute ethanol heating reflux method, condensing the extract to a certain volume, cooling and standing, filtering, extracting by the adding ethyl acetate into a filtrate, merging the extracts, condensing to a stiff paste, followed by vacuum drying of the stiff paste and crushing, redissolving by the use of water or an ethanol aqueous solution, performing vacuum drying and crushing to obtain the stilbene glucoside extract product. The technology preparation of the stilbene glucoside fine product comprises the following steps of: using the stilbene glucoside crude product as a raw material, separating by silica gel column chromatography, and purifying by Sephadex LH-20 column chromatography to obtain the stilbene glucoside fine product. The technology provided by the invention has advantages of simple operation, high recovery rate and good stability, and is suitable for industrial production.

Description

Technical field: [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a production process for extracting effective components of stilbene glycosides from Polygonum multiflorum, and a pharmaceutical preparation of stilbene glycosides. Background technique: [0002] Polygonum multiflorum is the dry tuber root of Polygonum Multiflorum Thumb. According to its processing method, it is divided into raw Shouwu and system Shouwu. Raw Shouwu is bitter, astringent, and flat in nature, and has the effects of moistening the intestines, detoxifying, and cutting off malaria. It is used for intestinal constipation, carbuncle, scrofula and other diseases. Shouwu is bitter, sweet, astringent, warm in nature, and has the effects of nourishing liver and kidney, nourishing essence and blood, and strengthening muscles and bones. Nocturnal emission embolism. The main components are hydroxyanthraquinone compounds and glycoside compounds, as well as some ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08A61K9/20A61K9/48A61K9/08A61K9/14A61K31/7028A61P3/06A61P39/06A61P35/00
Inventor 于永明刘成东徐明波窦学义高坤姜昊王俊玲袁宛君
Owner BEIJING SL PHARMA
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