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Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt

A technique of perilla frutescens extract and root nematodes, which is applied in the fields of botanical equipment and methods, chemicals for biological control, nematicides, etc., can solve the problems of high resistance to root nematodes that have not yet been seen, and achieve a significant effect of increasing production , good compatibility, the effect of reducing harm

Inactive Publication Date: 2012-10-17
HUNAN UNIV OF HUMANITIES SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disease-resistant varieties: In the study of the relationship between rice and root nematodes, there has been no report of a certain rice variety being highly resistant to root nematodes

Method used

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  • Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt
  • Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt
  • Method of mitigating harm of Hirschmanniella to paddy rice by using extract product of Perilla frutescens (L.) Britt

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Perilla frutescens (L.) Britt.] extract improves rice resistance to root nematode infection. The following examples are further descriptions of the present invention, and the following are the results of indoor bioassays, but they should not be regarded as limiting the scope of application of the present invention in any way.

[0018] Select the dried perilla herb, crush it at low temperature, and pass through a 20-50 mesh sieve; extract or extract the crushed perilla, use an organic solvent as an entrainer, such as ethanol, and use an amount of 10 times the total amount of the powder. The extract was concentrated by rotary evaporation in a water bath at 45°C under reduced pressure; (about 8g perilla can obtain 1g extract), and stored in a refrigerator at 4°C.

[0019] Put 200g of air-dried soil in a 13cm×8cm×5cm plastic box, add 100ml of natural water, soak the rice seeds at 25°C for 24 hours, and accelerate germination at 30°C for 12 hours. Boxes of 15 grains were pl...

Embodiment 2

[0025] Evaluation of field efficacy of perilla extract in alleviating root-root nematode damage to rice.

[0026] Extraction method: choose dried perilla herb, crush it at low temperature, and pass through a 20-50 mesh sieve; extract or extract the crushed perilla, use water as entrainer, such as distilled water, and use 15% of the total amount of powder. times, leached and filtered through a water bath at 60°C under reduced pressure for 3 times, collected the filtrate, concentrated it and then evaporated to obtain the extract; (about 9g perilla can get 1g extract), and stored in a refrigerator at 4°C.

[0027] Rice variety: Louyou 207

[0028] The base number of root nematodes in the experimental field: 7450 heads / 100 grams of roots (old roots of the previous season)

[0029] The paddy rice was sown on June 22, 2010, and transplanted when the seedlings grew to the four-leaf stage, and the chemical treatment was carried out 10 days after planting. , 32, 64g / m 2 Seven doses,...

Embodiment 3

[0034] Evaluation of field efficacy of perilla extract in mitigating root-root nematode damage to rice (root dipping).

[0035] Extraction method: choose dried perilla herb, crush it at low temperature, and pass through a 20-50 mesh sieve; extract or extract the crushed perilla, use an organic solvent as an entrainer, such as acetone, and use an amount of 1% of the total amount of powder 10 times, leached and filtered through a water bath at 40°C under reduced pressure for 3 times, collected the filtrate, concentrated and then rotated to evaporate to obtain the extract; (about 8g of perilla can get 1g of extract), and stored in a refrigerator at 4°C.

[0036] Rice variety: Tanliangyou 83

[0037] The base number of root nematodes in the experimental field: 4280 heads / 100 grams of roots (old roots of the previous season)

[0038] The paddy rice was sown on April 16, 2011, and transplanted when the seedlings reached the four-leaf stage. When transplanting, chemical treatment was ...

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Abstract

The invention specifically relates to a method of mitigating harm of Hirschmanniella to paddy rice by using an extract product of Perilla frutescens (L.) Britt, which belongs to the field of biological control technology for nematodiasis of paddy rice. The extract product of Perilla frutescens (L.) Britt is prepared by crushing and sieving perilla leaves, seeds and stalks and carrying out extraction and condensation with water or an organic solvent used as an entrainer. The extract product is diluted and then combined with a pesticide and a fertilizer, and then an obtained mixture is directly sprayed onto paddy rice, or roots of seedlings are dipped in the mixture during transplanting. The extract product of Perilla frutescens (L.) Britt can mitigate the harm that Hirschmanniella does to paddy rice, enables the purposes of yield increasing and income increasing to be achieved, is safe to beneficial organisms and poses no pollution to environment.

Description

field of invention [0001] The invention belongs to the technical field of rice nematode disease biological control, and in particular relates to a method for reducing the damage of root nematodes to rice by using perilla [Perilla frutescens (L.) Britt.] extract. More specifically, the present invention relates to perilla [Perilla frutescens (L.) Britt.] Use of Perilla frutescens (L.) Britt.] extract on rice. Background technique [0002] Rice root-potent nematode (Hirschmanniella spp) has become a global rice pest, and the diseases caused by it infecting rice lead to severe yield reduction and are difficult to control. The method of paddy-dry rotation can effectively reduce the occurrence of diseases, but there are not many varieties of crops to choose for rotation, and because rice is the daily staple food of people, long-term paddy-dry rotation reduces the area of ​​rice planting, which affects food security and social stability, so it is difficult to implement continuousl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/22A01P5/00A01G7/06
Inventor 金晨钟刘桂英
Owner HUNAN UNIV OF HUMANITIES SCI & TECH
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