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Preparation method, seed propageation method and application of seeds of rice male sterile line

A technology for male sterility and rice seeds, applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of application limitation, low reproduction yield, narrow temperature range for restoring fertility, etc., and avoid negative effect, simplification of production procedures, and avoidance of cytoplasmic homogeneity

Active Publication Date: 2012-10-17
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The emergence of this problem has seriously affected the application prospects of two-line hybrid rice, and is a major obstacle to the further promotion and application of two-line hybrid rice.
[0007] On the other hand, the temperature-sensitive CMS lines with low sterility threshold temperature are sterile in summer seed production, but the yield of their own reproduction is low and unstable, and sometimes even fails because they return to the fertile temperature. The range is very narrow, generally only about 3°C, so the application of this type of material is also greatly restricted

Method used

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  • Preparation method, seed propageation method and application of seeds of rice male sterile line
  • Preparation method, seed propageation method and application of seeds of rice male sterile line
  • Preparation method, seed propageation method and application of seeds of rice male sterile line

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, the production method of rice male sterile strain

[0031] 1. Obtaining of rice male sterile lines

[0032] The conventional rice material, japonica rice 9522 seeds, was used. use 60 Coγ-rays were irradiated with a treatment dose of 280Gy to obtain mutagenic materials.

[0033] A male sterile mutant in the F2 generation of the mutagenic material was backcrossed for three generations to obtain a stable genetic rice mutant osms4 regulated by a recessive single gene.

[0034] Afterwards, the rice mutant osms4 was backcrossed with japonica rice 9522, and all F1 generations were fertile. Segregation occurred in the self-crossed F2 generation, of which 419 were normal plants and 126 were mutant plants, and the ratio of normal plants to mutant plants was close to 3:1 (x 2 =1.028, P>0.05), indicating that the phenotype of the male sterile mutant was caused by a single nuclear gene mutation.

[0035] 2. Determine the mutation site of rice male sterile lines ...

Embodiment 2

[0058] Embodiment 2, rice male sterile line fertility conversion and breeding method

[0059] The fertility conversion of the rice osms4 mutant obtained in Example 1 can be realized by controlling the temperature and light environment. Under natural conditions, young panicle differentiation progresses to the stage of pollen mother cell formation, when the daily light is ≤13 hours, the fertility of the sterile line is stable, the anthers are white, and the pollen staining is normal;

[0060] When the daily light time is ≥ 13.5 hours, the number of light days is ≥ 2 days, and the temperature is 26-30°C, it will be converted from sterile to fertile. When the daily light time is ≥14 hours and the temperature is 25-35°C, it will be converted from sterile to fertile. The pollen dyeing normal pollen grains are 45-80%, and the seed setting rate is between 60-80%. The fertility transition of the rice osms4 mutant is sensitive to light duration, light intensity and spectral range. ...

Embodiment 3

[0061] Embodiment 3, the purposes of rice male sterile line in rice hybrid breeding

[0062]Autumn seed production in Shanghai in 2008, with an area of ​​1.0 mu; the male parent is the japonica restorer line Xiangqing (JP50) selected by the Shanghai Academy of Agricultural Sciences. The male parent was sown on May 30, transplanted on June 25, and transplanted on September 5. heading;

[0063] The female parent (ie the osms4 mutant of rice in Example 1) was sown on June 23, transplanted on July 12, and heading on September 8; the parent-to-parent ratio was 2:8.

[0064] On the first day of flowering of the female parent, the pollen was sampled for microscopic examination. 2 -IK staining: 80% pollen is normal, 20% is round; bagging self-fertilization is 0. The female parent blooms early, the flowering time is relatively concentrated, and the parental parental flowers meet well; the hybrid seeds are harvested 140kg; in the winter of 2008, Hainan's purity test showed that the ...

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Abstract

The invention relates to a preparation method, seed propageation method and application of seeds of a rice male sterile line. The preparation method comprises the following steps: mutating an OsMS4 gene sequence in the rice seeds by using routine methods, and acquiring a rice male sterile line with a mutation in homozygous recessive OsMS4 gene by traditional genetic hybridization screening. The OsMS4 sterile line can recover pollen fertility under certain seed propageation conditions, and has the capacity of propagating the seeds containing the mutation in the homozygous OsMS4 gene. The seeds containing the mutation in the homozygous OsMS4 gene can realize seed propageation. According to the invention, by controlling the rice cultivation time to regulate the reproductive growth of rice, the fertility and sterility of the rice male sterile line, that is, the OsMS4 plant can be controlled, one line with dual purposes can be realized, and the production process of hybrid rice seeds is simplified.

Description

[0001] This application is a divisional application with the application number 201010237721.6, the filing date is 2010.7.27, and the title of the invention is "Seed production, propagation and two-line hybrid breeding method based on rice-based osms4 mutant". technical field [0002] The present invention relates to a seed preparation method, seed propagation method and application thereof in the field of biotechnology, in particular to a preparation method, seed propagation method and application of rice male sterile line seeds. Background technique [0003] Rice male sterile line refers to a female parent rice material with abnormal development or degeneration of male reproductive organs (mainly degeneration of anthers or pollen) but normal development of pistil reproductive organs. Due to abnormal early development of anthers or pollen, pollenless or asexual Vitality pollen cannot be self-pollinated and fruited, and can only be fertilized and fruited by relying on foreign...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06
Inventor 张大兵梁婉琪袁政张辉
Owner SHANGHAI JIAO TONG UNIV
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