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Hybridization system for real-time monitoring parsing process of micro array chip

A kind of microarray chip, technology of analysis process

Active Publication Date: 2014-07-09
BOAO BIOLOGICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the signal changes on the chip surface cannot be monitored in real time, this kind of control experiment consumes a lot of samples and time

Method used

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  • Hybridization system for real-time monitoring parsing process of micro array chip
  • Hybridization system for real-time monitoring parsing process of micro array chip
  • Hybridization system for real-time monitoring parsing process of micro array chip

Examples

Experimental program
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Embodiment Construction

[0021] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0022] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0023] Such as figure 1 As shown, the hybridization system provided by the present invention includes a hybridization cavity, which is composed of a microarray chip 1, a cavity cofferdam 2 and a cover slip 3; the hybridization cavity is connected with a temperature control device 4 for Realize the temperature control of the hybridization cavity; the inlet of the hybridization cavity is connected with an air bottle 5, a sample bottle 6 and two washing liquid bottles 7 and 8 respectively; the pipeline that the hybridization cavity communicates with the air bottle 5 A pinch valve 91 is provided, a pinch valve 92 is provided on the pipeline connecting the hybridization chamber and the sample bottle 6, and a pinch valve 93 is respec...

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Abstract

A system for real-time monitoring of a microfluidic reaction and a method thereof are provided. The system comprises a platform for holding a microfluidic device; a temperature control module (4); a peripheral flow module; and a real-time imaging module. The method comprises providing a reagent to the microfluidic device; and monitoring the reaction using the real-time imaging module. Furthermore, methods for optimizing a reaction condition using the system are provided.

Description

technical field [0001] The invention relates to a hybridization system, in particular to a hybridization system for real-time monitoring of the microarray chip analysis process. Background technique [0002] Microarray chips are formed by immobilizing a large number of probe molecules with known sequences on the support in an orderly and high-density manner by means of micro-processing technology. The probe molecules can be DNA, RNA, protein, sugar, etc. By detecting whether the probe hybridizes with the target substance in the sample, gene expression characteristics, genetic defects, protein distribution, reaction characteristics, etc. can be analyzed. [0003] The analysis method based on microarray chips mainly includes steps such as sample pretreatment, chip preparation, chip hybridization, chip cleaning and drying, and chip detection. The current chip analysis platform includes two types: one type of platform uses large-scale equipment to complete processing steps such...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34G01N33/68
CPCG01N33/68G01N33/54366B01L7/52B01L3/502715B01L2400/0487
Inventor 朱疆郭洪菊梅丹阳宋秀梅王东程京
Owner BOAO BIOLOGICAL CO LTD
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