Moth grinding sample preparation system for detecting silkworm female moth corpuscule pathogeny spore

Abstract

The present invention relates to a moth grinding sample preparation system for detecting microparticle pathogenic spores of Bombyx moth moth, which consists of moth grinding system 1, automatic liquid transfer system 2, centrifugal enrichment device 3, centrifugal tube washing device 4 and automatic cleaning system 5 components, and in turn integrated in a worktop. The present invention basically realizes the mechanical operation of each link of grinding moth sample preparation, saving labor and labor; the liquid injection and the grinding moth are carried out synchronously, which increases the chance of contact between the material and the blade, and the material is more thoroughly ground; the electromagnetic automatic pipetting (suction liquid return), the liquid return volume is uniform and accurate; the automatic transmission spray washing device is used to wash the centrifuge tube, covering the grinding moth, suction liquid return, centrifuge tube washing, and automatic spray washing is realized. Using high-pressure automatic flushing appliances, the water-saving effect is very significant, and 80% of water consumption can be saved. It has the advantages of fast work rate, water saving, good safety performance and high sample clarity. It can be used in silkworm egg production units and silkworm egg quality inspection departments to quickly prepare large batches of samples when microscopic detection of microscopic pathogenic spores of silkworm female moths.

Description

technical field [0001] The invention relates to the technical field of agricultural machinery, in particular to a moth sample preparation system for detecting microparticle pathogenic spores of Bombyx moth moths. Background technique [0002] Bombyx mori microparticle disease is a contagious silkworm disease that is highly harmful to sericulture. In the middle of the nineteenth century (1845-1865), it brought European sericulture to the brink of extinction. From 1865 to 1870, microbiologist Pasteur found that Microparticle pathogenic spores have the characteristics of embryo infection, that is, the characteristics of infecting and harming offspring from the previous generation of female moths. The female moth inspection technology of single moth egg production, single moth inspection, and removal of diseased eggs has been invented. This technology has saved silkworms in Europe and the world Industry. With the expansion of production scale, the number of inspection samples i...

AI Technical Summary

Problems solved by technology

At present, the process of using moth grinding machine to prepare samples is to put the sampled female moth into the material cup, and then go through the process of liquid injection, moth grinding, standing, filtering, centrifugal sedimentation and shaking spotting. Except for grinding moths, other operations are It is labor-intensive to complete manually, and it is impossible to quickly extract multiple samples, and the material cups and centrifuge tubes are rinsed with tap water manually after use, which consumes a lot of water and is extremely wasteful.

So far, the preparation of samples for the detection of microparticle pathogenic spores of silkworm egg production female moths has only stopped at the stage of mechanically grinding female moths, and there is no new research progress at home and abroad on the mechanical development and system integration of the subsequent operation process

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