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Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses

A technology of Yarrowia lipolytica and production method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inappropriate lipase production, and achieve the effect of promoting application

Active Publication Date: 2012-10-03
LABES MAYOLY SPINDLER R L
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] Prior art methods of producing lipase, even if they make it possible to obtain increased lipase yields, are not suitable for the production of lipase suitable for medical purposes

Method used

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  • Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses
  • Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses
  • Method for producing lipase, transformed Yarrowia lipolytica cell capable of producing lipase and their uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 - Materials and methods

[0064] 1) Medium used

[0065] The final concentrations shown are those in the stock solutions.

[0066] Unenriched Basal Medium 02130S

[0067] Element final concentration glucose 10g / l K H 2 PO 4 3g / l Na 2 HPO 4 3g / l h 3 BO 3 0.34g / l (NH 4 ) 2 SO 4 3g / l C 5 h 8 o 4 NNa (glutamic acid) 1g / l MgSO 4 0.5g / l CaCl 2 0.023g / l MnSO 4 0.038g / l ZnSO 4 0.04g / l

[0068] Additional Enrichment Medium 02130S contains the following supplements:

[0069] additive final concentration trace element solution 1ml / l vitamin solution 1ml / l

[0070] Trace element solution formula

[0071] Element final concentration CoCl 2 0.5g / l Na 2 MoO 4 0.06g / l CuSO 4 0.9g / l

[0072] vitamin solution formula

[0073] Element final concentration D-biotin 0.05g / l ...

Embodiment 2

Embodiment 3

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Abstract

A method for producing Yarrowia lipolytica acid-resistant recombinant lipase utilizes a culture medium without any products of animal origin or non-characterized mixtures such as tryptone, peptone or lactoserum. A recombinant strain of Yarrowia lipolytica capable of producing an excessive amount of the lipase Lip2 is referred to as YL-LIP2-6C and filed with the Collection Nationale de Cultures de Microorganismes (C. N. C. M.) under a number of I-3542 on December 15 2005. The invention further relates to uses of the recombinant strain.

Description

[0001] This application is a divisional application of the invention patent application with the filing date of June 15, 2006 and the application number of 200680054940.2 (PCT / FR2006 / 001352). technical field [0002] The present invention relates to a method for the production of lipase by using Yarrowia lipolytica cells producing an acid-resistant recombinant lipase, which method allows the production of lipase which can be used as a medicine; the present invention also relates to a A strain of Yarrowia lipolytica that overproduces an acid-tolerant recombinant lipase and its use. Background technique [0003] The daily food intake of human beings is mainly composed of lipids, proteins and sugars. Before they are absorbed, all of these components are subjected to enzyme-catalyzed hydrolysis in the digestive tract. Deficiency in any of these enzymes can thus cause digestive disturbances and lead to considerable malnutrition. That is, for example, pancreatic lipase deficienc...

Claims

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Application Information

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IPC IPC(8): C12N9/20C12N1/19C12R1/645
Inventor 伊夫·勒布隆尼古拉斯·穆兹阿兰·马蒂让·路易·乌里韦拉雷亚
Owner LABES MAYOLY SPINDLER R L
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