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Dibutyl phthalate degrading bacteria and application of dibutyl phthalate degrading bacteria

A technology of dibutyl phthalate and degrading bacteria, which is applied in the field of bioaugmentation of environmental pollutants, can solve the problems of low degradation efficiency of dibutyl phthalate, achieve good application prospects, high-efficiency degradation, and good biological The effect of enhancing the effect

Inactive Publication Date: 2012-10-03
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of low degradation efficiency of dibutyl phthalate (DBP) by ordinary microorganisms, and aims to provide a strain of DBP-degrading bacteria to realize its bioaugmentation of oil refining wastewater, pesticide wastewater, beer wastewater and coking wastewater deal with

Method used

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  • Dibutyl phthalate degrading bacteria and application of dibutyl phthalate degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Elizabeth Kingia Isolation, purification, identification and degradation characteristics of sp. D8 to DBP

[0023] 1. Isolation and purification of strains

[0024] (1) Source of bacteria

[0025] The source of the bacteria came from the activated sludge in the sewage treatment plant of Wuhan Branch of China Petroleum & Chemical Corporation.

[0026] (2) Isolation and purification of strains

[0027] Put the collected samples in a refrigerator at 4°C and inoculate 1 mL of the supernatant into 100 mL of sterilized LB medium (1 wt% peptone, 1 wt% sodium chloride, 0.5 wt% yeast extract). 30°C, 150r / min activation culture overnight. Then transfer 1 mL of the bacterial solution to the inorganic salt medium containing 200 mg / L DBP, acclimatize and cultivate for 2 days under the same conditions, gradually increase the concentration of DBP (400 mg / L, 600 mg / L, 800 mg / L , 1000 mg / L, 2000 mg / L). After the acclimatization is over, the bacteria solution is divi...

Embodiment 2

[0040] Example 2: environmental conditions Elizabeth Kingia Effect of sp. D8 degradability

[0041] 1. The influence of culture temperature

[0042] When the pH is 7.0, the shaker speed is 150 r / min and the inoculum size is 2 vol %, the effect of temperature on the degradation of DBP is as follows: figure 2 Shown: In the range of 20-40 ℃, the strain D8 has a better degradation effect on DBP, indicating that the strain has a wider adaptability to temperature. When the temperature is between 20 and 35°C, the degradation rate of the substrate increases as the temperature increases; but when the temperature reaches 40°C, the degradation rate of the substrate by strain D8 is lower than that at 35°C . show Elizabeth Kingia The optimum temperature for sp. D8 is 35°C.

[0043] 2. The influence of initial pH

[0044] When the temperature was 30°C, the rotation speed of the shaker was 150 r / min, and the inoculum size was 2 vol%, the effect of pH on the degradation of DBP wa...

Embodiment 3

[0049] Example 3: Elizabeth Kingia Sp.D8 enhanced treatment of refinery wastewater with higher concentration of DBP added

[0050] In the Erlenmeyer flask, the strain D8 was added to the activated sludge of Wuhan Petrochemical, and three groups of experiments were set up to investigate its removal effect on DBP:

[0051] (a) 30mL of oil refinery wastewater+33mL of DBP inorganic salt medium+400mg / L of DBP+30mL of activated sludge+7 vol% D8 bacterial suspension;

[0052] (b) 30mL of oil refinery wastewater+33mL of DBP inorganic salt medium+400mg / L of DBP+30mL of activated sludge after autoclaving+7 vol % of the D8 bacterial suspension;

[0053] (c) 30mL of refinery wastewater + 40mL of DBP inorganic salt medium + 400mg / L of DBP + 30mL of activated sludge.

[0054] Incubate on a shaker at 35°C and 150 rpm. At regular intervals, measure the residual concentration of DBP in the culture medium, such as Figure 6 As shown, the results show that: Elizabeth Kingia The activa...

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Abstract

The invention discloses a strain of dibutyl phthalate degrading bacteria and application of the dibutyl phthalate degrading bacteria. The strain of dibutyl phthalate degrading bacteria D8 provided by the invention belongs to Elizabethkingiasp and is preserved in the China Center for Type Culture Collection, wherein the preservation date is March 23 2012; the preservation number is CCTCCNO:M2012088, and the GenBank accession number of a bacterial strain 16SrDNA is JQ673498. The bacterium is Gram-negative bacterium; the bacterial colony is yellow, round, smooth and humid; the brim is regular, and the centre protrudes. The bacterium utilizes dibutyl phthalate as sole carbon source and energy source to grow and breed. The bacterium can completely degrade 400mg / L of DBP (dibutyl phthalate) within 60 hours when being purely cultivated. The bacterial strain D8 can tolerate DBP of which the concentration can be up to 2000mg / L, and has excellent degradation effect on high-concentration DBP industrial wastewater. The bacterium is applied to bioaugmentation treatment of refinery waste water, pesticide wastewater, brewery wastewater and coking wastewater, and has excellent application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant bioaugmentation, and in particular relates to a dibutyl phthalate degrading bacterium and application thereof. Background technique [0002] Dibutyl phthalate (DBP) is an important plasticizer widely used in wire and cable, furniture, automobile, clothing, cosmetics and other industries. DBP is an environmental hormone substance that can interfere with the reproductive system and development of animals and humans, and has developmental and embryotoxicity. Therefore, the U.S. Environmental Protection Agency, the European Union, and the National Environmental Monitoring Center of China all list DBP as a priority pollutant. [0003] Dibutyl phthalate plasticizers are easy to diffuse into the natural environment through different channels, and DBP is very stable in the natural environment, the rate of hydrolysis and photolysis is very slow, and its hydrolysis half-life is as long as 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/34
Inventor 颜家保王巧凤魏鑫游海许龙龙
Owner WUHAN UNIV OF SCI & TECH
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