Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish

Abstract

The invention relates to a breeding method and application of a cytoplasmic male sterility restoring line of brassica napus rapeseed and radish. The method comprises the following steps of: choosing rapeseed S1628 with the preservation number of CCTCC NO: P201203, performing continuous inbreeding directional selection for six years to breed an excellent, high-oil and stable inbred line; using the cytoplasmic male sterility restoring line of the brassica napus rapeseed and radish as a female parent and using the bred excellent and stable inbred line as a male parent to select out strains comprising radish cytoplasmic male sterility restoring genes from large amounts of filial generations; using the strains comprising the radish cytoplasmic male sterility restoring genes as the female parent and using Zhongshuang No.2, Huashuang No.3, Huyou 17, Zunyou No.1 and Zheyou 758 of double-low winter rapeseed varieties in China as recurrent male parents to hybridize, performing the continuous inbreeding directional selection for six years to breed the cytoplasmic male sterility restoring line of the double-low and high-oil brassica napus rapeseed and radish. The restoring line has been crossed with hybrid varieties. A gap of producing the hybrid varieties by the cytoplasmic male sterility restoring line of the double-low radish in China is filled, and the application prospect is broad.

Description

technical field [0001] The invention relates to the selection and breeding of a restorer line of three-line hybrid rapeseed, in particular to the selection and application of a cytoplasmic male sterile restorer line of Brassica radish. Background technique [0002] At present, the rape CMS with the most practical application value in the world is the Polyma CMS and the Shan 2A CMS. A series of rapeseed hybrids have been successfully selected using the Polyma CMS and the Shan 2A CMS. But these two kinds of sterile lines all have the problem of temperature sensitivity, that is to say, the fertility of these two sterile lines is unstable at low temperature, and both produce a certain amount of fertile pollen, and the sterile lines are easy to self-fertilize. The generated hybrid seeds have more than 10% sterile plants, the purity of the seed production is affected to a certain extent, and the yield of the hybrid cannot fully represent itself. [0003] Nuclear male sterile line...

AI Technical Summary

Problems solved by technology

Li Xufeng from Sichuan Union University, etc. used chromosome engineering to introduce the Ogucms restorer gene in the radish variety Orchid chinensis into rapeseed. Fertility has been well restored. This is the first Ogucms restoration source found from the Asian radish genome, but it has not been used in production so far.

Brassica napus radish cytoplasmic male sterile three-line hybrids have not been successfully applied to production so far. There are no reports on the application of double-low radish cytoplasmic male sterile restorer lines in China, and there are no related similar applications