Method for rapid determination of trans-fatty acid in food
A technology of trans fatty acid and determination method, which is applied in the field of food testing and can solve the problems such as the difficult realization of prerequisite requirements
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[0022] (1) Preparation of samples
[0023] Grease samples: Grease samples can be directly measured.
[0024] Oil-containing samples: According to the type of samples, extract fat according to GB / T 5009.6, GB / T 5009.46, GB / T 5413.3 or GB / T 9695.7 respectively, and then measure it.
[0025] (2) Determination
[0026] 1 Instrument conditions
[0027] Wave number range: 1050cm -1 ~900cm -1 .
[0028] Resolution: 4cm -1 .
[0029] Number of scans: 4 times.
[0030] 2 Standard curve creation
[0031] 2.1 Preparation of standard series samples
[0032] Accurately weigh three elaidic glycerides X g and triolein (0.3-X) g in a 5ml beaker, heat in a water bath at 60°C, and mix well, where X is 0.00000, 0.00150, 0.00300, 0.00600, 0.00900, 0.01500, 0.03000, 0.06000, 0.12000, 0.15000, 0.18000, the content of trieline olein is 0%, 0.5%, 1%, 2%, 3%, 5%, 10%, 20%, 40%, 50%, 60% for standard series samples.
[0033] 2.2 Collection of infrared absorption spectrum
[0034] Suck an a...
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