Fast cauliflower genetic purity detection method based on polymerase chain reaction (PCR) technology

Abstract

The invention discloses a fast cauliflower genetic purity detection method based on the polymerase chain reaction (PCR) technology. The method uses cauliflower hybrids of 100-day oriental snow as study objects, the screening of molecular markers is carried out, and seedling blade genome deoxyribose nucleic acid (DNA) and special primer combinations SREM8-1 / SRodd58 capable of simultaneously generating male parent and female parent special strip markers in the hybrids can be verified. Through the adoption of the primer, the method has the advantages that the accuracy is high, the results are stable, the influence caused by environment conditions and development period is avoided, the counting is simple and convenient, and the like, the seed genetic purity of the cauliflower hybrids of 100-day oriental snow can be simply, fast and accurately detected, the efficient and accurate quality control of cauliflower seeds is favorably realized, and the quality detection process of the cauliflower seeds is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rapid detection method for the genetic purity of cauliflower based on PCR technology. Background technique [0002] Cauliflower, Brasica oleraceae .var. botyris L., also known as cauliflower and cauliflower, is an important vegetable of the Brassica family, and is widely grown in China, India, Italy, Europe, the United States and other parts of the world. The edible part of cauliflower is made up of numerous shortened and fat flower branches and buds called "curds". Cauliflower is rich in nutrients. In addition to protein, cellulose and various minerals, it also contains a variety of indole derivatives, which have anti-cancer effects and have been listed as anti-cancer vegetables in recent years. In recent years, the production of cauliflower in my country has developed rapidly. my country is not only the country with the largest cauliflower planting area and the highe...

AI Technical Summary

Problems solved by technology

Due to some objective reasons, such as the parent is not a pure line, complex ecological conditions in the seed production area, etc., as well as some human factors, such as counterfeit and shoddy products, incidents of pitting and harming farmers, and sales of seeds with unqualified purity, many agricultural production accidents and groups have been caused. The incident brought serious losses and negative impacts to vegetable producers and seed sales enterprises

In the process of cauliflower F1 generation hybrid seed production, self-crossed seeds of the female parent line, foreign pollen, and pseudo-hybrids produced by mechanical mixing are often mixed in F1, which seriously affects the quality and purity of seeds, and brings huge economic losses to farmers. , bringing claim risks to seed producers and operators

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