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A composite structure artificial blood vessel and its dynamic preparation method

A technology of artificial blood vessels and composite structures, which is applied in the fields of blood vessels, human tubular structure devices, medical science, etc. Controllable layer quantity and good adhesion

Active Publication Date: 2016-06-08
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to my country's intellectual property system, there are currently existing intellectual property projects that reduce the formation of thrombus by modifying the surface of the inner wall of blood vessels. The composite artificial blood vessel composed of fabric reinforcement layer and elastic adhesive layer can improve the patency rate of artificial blood vessel to a certain extent; however, elastic adhesive layer is needed to connect the fabric reinforcement layer and anticoagulant layer, resulting in thicker tube wall, It is not conducive to the application of small-diameter artificial blood vessels, and its structure is complex, difficult to prepare, and the amount of coating is uncontrollable

Method used

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  • A composite structure artificial blood vessel and its dynamic preparation method
  • A composite structure artificial blood vessel and its dynamic preparation method
  • A composite structure artificial blood vessel and its dynamic preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The material of the tube blank adopts 50D / 12f viscose glossy yarn; the warp and weft yarn structure is three-ply joint knitting; Figure 5 Shown is woven from ground warp 6, twisted warp 7, and weft yarn 8). Fabric parameters: warp density * weft density (threads / 10cm) is 400*100, folding diameter (mm) is 10, and total warp number (roots) is 40.

[0043] Weaving organization chart see Figure 4 . The cellulose fermented by Acetobacter xylinum is selected to coat the tube blank. in the bioreactor ( figure 1 with 2 ) for 1-7 days to ferment and culture, and finally prepare bacterial cellulose-coated small-diameter artificial blood vessels.

[0044] Fermentation culture process:

[0045] (1) Strain cultivation: insert Acetobacter xylinum into 300mL liquid medium (per 1L of water, 20g of mannitol, 3g of peptone, 5g of yeast extract, pH 3.0, sterilized at 121°C for 20min; or 200g of glucose , yeast extract 5g, tryptone 5g, citric acid 115g, Na 2 HPO 4 2.7g, 1L of wa...

Embodiment 2

[0048] The material of the tube blank is 50D / 12f viscose glossy yarn; the yarn structure is three-ply joint knitting; Fabric parameters: warp density * weft density (pieces / 5cm) is 35*55, cylinder diameter (mm) is 6.

[0049] Weaving organization chart see Image 6 . Cellulose fermented by Gluconobacteroxydans is selected to coat the tube blank. in the bioreactor ( figure 1 with 2 ) for 1-7 days to ferment and culture, and finally prepare bacterial cellulose-coated small-diameter artificial blood vessels. Electron micrographs of artificial vascular materials coated with nanocellulose and image 3 The results are similar.

[0050] Fermentation culture process:

[0051] (1) Strain cultivation: Insert Gluconobacteroxydans into 300mL liquid medium (per 1L of water, mannitol 20g, peptone 3g, yeast extract 5g, pH 3.0, sterilized at 121°C for 20min; or glucose 200g , yeast extract 5g, tryptone 5g, citric acid 115g, Na 2 HPO 4 2.7g, 1L of water, pH 7.5, sterilized at 121°C f...

Embodiment 3

[0054] The yarn of the tube blank is made of 50D / 12f viscose glossy yarn; the basic structure is a rope-knitted tubular structure. Fabric parameters: the number of bobbins is 32, and the diameter (mm) of the core tube is 6.

[0055] Cellulose fermented by kombucha is selected to coat the tube stock. in the bioreactor ( figure 1 with 2 ) for 1-7 days to ferment and culture, and finally prepare bacterial cellulose-coated small-diameter artificial blood vessels. Electron micrographs of artificial vascular materials coated with nanocellulose and image 3 The results are similar.

[0056] Fermentation culture process:

[0057] (1) Strain cultivation: put black tea fungus (kombucha) into 300mL liquid seed culture medium according to the inoculation amount of 5 discs with a diameter of 0.5cm containing bacteria BC membrane (every 1L of water, green tea 5g, glucose 10, peptone 3g , Yeast extract 5g, pH3.0, pasteurized for 30min; per 1L of water, glucose 100g, peptone 3g, yeast e...

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Abstract

The present invention relates to an artificial blood vessel with a composite structure and a dynamic preparation method thereof. The artificial blood vessel comprises: a textile-based tube blank (1) and a nanocellulose coating (9); the preparation method includes: weaving, knitting, braiding or Non-woven technology such as spunlace obtains the tube blank (1) of the artificial blood vessel, then the tube blank is placed in the bacterial cellulose bioreactor, the tube blank (1) is passed through the bracket (2), and the bracket (2) There is a gap between it and the tube blank (1). After high-temperature sterilization, pour sterile fermentation medium into the reactor, insert bacterial cellulose production bacteria seed liquid, and coat the inner and outer surfaces of the tube blank after dynamic culture. A layer of nanocellulose film is obtained. The small blood vessel of the present invention has good tensile properties in the radial and axial directions, the thin tube wall meets the requirements of small-caliber blood vessels, the tube wall has good airtightness, and does not require pre-coagulation; Compatibility and biomechanical properties are good.

Description

technical field [0001] The invention belongs to the field of artificial blood vessels and their preparation, in particular to an artificial blood vessel with a composite structure and a dynamic preparation method thereof. Background technique [0002] At present, large-caliber vascular grafts have achieved good results in clinical applications, but ordinary small-caliber artificial blood vessels have almost no antithrombotic properties. [0003] According to my country's intellectual property system, there are currently existing intellectual property projects that reduce the formation of thrombus by modifying the surface of the inner wall of blood vessels. The composite artificial blood vessel composed of fabric reinforcement layer and elastic adhesive layer can improve the patency rate of artificial blood vessel to a certain extent; however, elastic adhesive layer is needed to connect the fabric reinforcement layer and anticoagulant layer, resulting in thicker tube wall, It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/06A61L27/34A61L27/50
CPCA61L27/507
Inventor 洪枫李毓陵王聪唐水佳陈琳
Owner DONGHUA UNIV
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