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Layer-by-layer electrostatic self-assembling method based on dopamine-modified polyelectrolyte and application

An electrostatic self-assembly and polyelectrolyte technology, which is applied in the field of surface modification of biomedical materials, can solve the problems of simplicity and universality discount of layer-by-layer assembly technology, and achieves elimination of substrate treatment, widening of application fields, and easy operation. Effect

Inactive Publication Date: 2012-08-15
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different materials often require different substrate pretreatments, which greatly reduces the simplicity and universality of layer-by-layer assembly technology

Method used

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  • Layer-by-layer electrostatic self-assembling method based on dopamine-modified polyelectrolyte and application
  • Layer-by-layer electrostatic self-assembling method based on dopamine-modified polyelectrolyte and application
  • Layer-by-layer electrostatic self-assembling method based on dopamine-modified polyelectrolyte and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This example is a layer-by-layer electrostatic assembly of multilayer films based on dopamine-modified polyelectrolytes on the surface of titanium alloys, without pretreatment on the surface of the material, that is, the surface of the material does not need to be pre-charged.

[0022] First, dopamine-modified hyaluronic acid was synthesized, and 0.1 g of hyaluronic acid was dissolved in 10 mL of PBS solution with pH=5.5. Add 40 mg of carbodiimide (EDC) into the solution, react for 15 minutes, add 60 mg of N-hydroxysuccinimide (NHS) and 50 mg of dopamine and react in the dark for 12 hours. During the reaction, use 1M HCl or 1M NaOH to maintain the solution pH=5.5, The solution was dialyzed and freeze-dried. Its electrostatic assembly process includes the following steps, and the material is titanium alloy:

[0023] 1) Dissolving dopamine-modified hyaluronic acid in NaCl aqueous solution with pH=5, and preparing a solution with a concentration of 1 mg / mL;

[0024] 2) d...

Embodiment 2

[0034] This example is a layer-by-layer electrostatic assembly of multilayer films based on dopamine-modified polyelectrolytes on the surface of hydroxyapatite, which does not require pretreatment on the surface of the material, that is, the surface of the material does not need to be charged in advance.

[0035] First, dopamine-modified sodium alginate was synthesized by dissolving 0.1 g of sodium alginate in 10 mL of PBS solution with pH=5.5. Add 40 mg of EDC to the solution, react for 15 minutes, add 60 mg of NHS and 50 mg of dopamine and react in the dark for 20 hours. During the reaction, use 1M HCl or 1M NaOH to maintain the pH of the solution at 5.5, dialyze the solution and freeze-dry.

[0036] Its electrostatic assembly process includes the following steps, and the material is hydroxyapatite:

[0037] 1) Dissolving dopamine-modified sodium alginate in NaCl aqueous solution at pH=5, and preparing a solution with a concentration of 1 mg / mL;

[0038] 2) dissolving chito...

Embodiment 3

[0048] This example is a layer-by-layer electrostatic assembly of multilayer films based on dopamine-modified polyelectrolytes on the surface of polytetrafluoroethylene, without pretreatment on the surface of the material, that is, the surface of the material does not need to be charged in advance.

[0049]Firstly, dopamine-modified gelatin was synthesized, and 0.1 g of gelatin was dissolved in 10 mL of PBS solution with pH=5.5. Add 40 mg of EDC to the solution, react for 15 minutes, add 60 mg of NHS and 50 mg of dopamine and react in the dark for 24 hours. During the reaction, use 1M HCl or 1M NaOH to maintain the pH of the solution at 5.5, dialyze the solution and freeze-dry.

[0050] Its electrostatic assembly process includes the following steps, and the material is polytetrafluoroethylene:

[0051] 1) Dissolving dopamine-modified gelatin in NaCl aqueous solution with pH=5, and preparing a solution with a concentration of 1 mg / mL;

[0052] 2) dissolving chitosan in an aqu...

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Abstract

The invention discloses a layer-by-layer electrostatic self-assembling method based on dopamine-modified polyelectrolyte and application. Dopamine and polyelectrolyte with carboxyl are subjected to dark reaction through carbodiimide / N-hydroxysuccinimide (EDS / NHS) so as to generate the polyelectrolyte of which a carboxyl part of a main chain is substituted by a pyrocatechol functional group; two hydroxyl groups on a benzene ring of pyrocatechol form tight combination with the material surface; and the obtained polyelectrolyte is alternatively deposited together with the polyelectrolyte with opposite charges so as to be assembled into a multi-layer film. Compared with other layer-by-layer assembling techniques, the layer-by-layer electrostatic self-assembling method disclosed by the invention has the advantages that being simple in operation, not affected by material and shape of a substrate, free of surface pretreatment, high in binding strength with the substrate and better in popularization and application value.

Description

technical field [0001] The present invention relates to electrostatic layer-by-layer self-assembly technology, more specifically, to a layer-by-layer self-assembly method based on dopamine modification, which is mainly used for surface modification of biomedical materials, such as functional metal ion loading, drug-loaded controlled release, Growth factor delivery and other fields. Background technique [0002] Layer-by-layer self-assembly technology is a supramolecular self-assembly technology based on polyelectrolyte anions and cations, which has the characteristics of simple operation, no substrate shape requirements, and stable film layers. However, the electrostatic layer-by-layer assembly technology first requires a charged substrate, and the distribution and existence of charges on the substrate directly affect the structural stability of the film. How to charge the uncharged substrate is a key issue in layer-by-layer assembly technology. The current substrate treat...

Claims

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Application Information

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IPC IPC(8): C23C26/00C08H1/00C08B37/08C08B37/04C23C22/54C08J7/12C08L27/18
Inventor 张鑫明李朝阳原续波崔振铎杨贤金
Owner TIANJIN UNIV
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