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Tissue culture and rapid propagation method of salt-tolerant field mint

A tissue culture rapid propagation, salt-tolerant technology, applied in the field of agricultural biology

Active Publication Date: 2012-08-15
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Salt-tolerant mint has been asexually propagated for a long time, which is prone to viral diseases, causing variety degradation, yield and quality decline, and resulting in a shortage of salt-tolerant mint germplasm resources

Method used

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  • Tissue culture and rapid propagation method of salt-tolerant field mint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1. The establishment of the explant sterile line adopts the method that the stem sections of different growth stages are sterilized separately

[0030] Take the vigorously growing mint plants from the nursery, remove the leaves and leave the stems and buds, rinse with running water for 20 minutes, absorb the water with filter paper, trim the explants with surgical scissors, cut off 3 cm long stems, and then divide the Stem segments (stem tip, middle stem segment, base stem segment) are separated and set aside. After sterilizing with 75% alcohol solution for 10s-60s and 0.1% mercuric chloride solution for 8-15 minutes on the ultra-clean workbench, rinse it with sterile water for 4-5 times, blot the water and then use surgical scissors to clean the explants. Trim, remove the black parts at both ends, then inoculate it in 1 / 2MS medium, and place it in a light incubator for cultivation. After 3 days, the pollution situation of the plants was observed, and the po...

Embodiment 2

[0032] Embodiment 2: Tissue culture formula screening

[0033] 2.1 Screening of callus induction medium (see Table 2, Table 3).

[0034] Orthogonal test L4(2 3 )filter. Select 3 cm long robust sterile seedlings and place them in the culture medium to induce callus. After 30 days of culture, the callus induction rate was investigated. Callus induction rate = number of stem callus / number of inoculated stems × 100%.

[0035] It can be seen from Table 3 that the orthogonal optimal formula is A (1,2) B 1 C 2 , namely 1 / 2MS or 2 / 3MS, 1.0 mg·L - 1 6-BA, 0.2mg·L - 1 NAA, 30g L -1 Sucrose and 4 g·L -1 GEL. It can be seen from the R value that NAA (C) is an important factor, 6-BA (B) is also a more important factor, and MS (A) is a less influential factor, that is, the influencing factor C>B>A. Therefore, this test determined that NAA was an important factor in the ability of salt-tolerant peppermint to induce callus, and its optimal concentration was 0.2 mg·L - ...

Embodiment 3

[0043] Example 3: Screening of salt-tolerant variants (see Table 6)

[0044] Take healthy rootless seedlings with a length of 2 cm and inoculate them into the rooting medium added with 0.1%, 0.3%, 0.5%, 0.8% and 1.0% sodium chloride respectively by weight, and investigate the rooting situation after three weeks.

[0045]

[0046] It can be seen from Table 6 that the addition of a salt concentration below 0.5% in the rooting medium does not affect the normal rooting of salt-tolerant mint. However, when the salt concentration was above 0.8%, the rooting rate dropped to 45%, the number of roots became significantly smaller, and the length of the roots became significantly shorter.

[0047] It shows that the optimal range of sodium chloride is 0.5% to 0.8% in the process of salt tolerance mutagenesis.

[0048]

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Abstract

The invention discloses a tissue culture and rapid propagation method of a salt-tolerant field mint and relates to a tissue culture and rapid propagation technology in the field of an agricultural biotechnology. The technical key points are as follows: the method comprises the following steps of: establishing an explant sterile system by utilizing a method for separately sterilizing stem sections in different growth periods; screening a callus induction culture medium; screening an adventitious bud propagation culture medium; screening a root-taking culture medium; screening a salt-tolerant variant; and carrying out acclimatization and transplanting method of tissue culture seedlings. Compared with the prior art, the method adopts the way for separately sterilizing different stem sections to completely disinfect and prevent disinfecting liquid from damaging the explants in the different growth periods; a culture medium formula in each culture phase is optimized, so that the salt-tolerant field mint is convenient for industrial production and has a high propagation coefficient; and the variants can be screened by oriented induction of the tissue culture phase so as to obtain a high-salt-tolerant product.

Description

technical field [0001] The invention relates to a technique for tissue culture rapid propagation of salt-tolerant mint, and the invention belongs to the field of agricultural biotechnology. Background technique [0002] Soil salinization is a major problem that plagues agricultural production. Currently, the world's irrigated land area is about 230 M hm 2 , of which the soil affected by salt damage accounts for about 45M hm 2 . Dryland agricultural area is about 1500M hm 2 , of which the soil affected by salt damage accounts for about 32M hm 2 . The degraded area of ​​cultivated land soil salinization is increasing at the rate of 3 hm 2 / min speed increase. According to FAO estimates, worldwide, the annual loss of land productivity due to salt damage reaches 0.25-0.50 M hm 2 . See Wang Xiaobin, focusing on the overlapping effects of water resource use and climate change on soil salinization (China Soil and Fertilizer, 2009(2): 80). As far as our country is concerne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨合同李纪顺王贻莲魏艳丽陈凯郭凯
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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