Collection device for collecting cells after laser microdissection, method and system thereof

Abstract

The invention discloses a collection device for collecting cells after laser microdisssection, a method and a system thereof. The device provided by the invention comprises a sample carrier, two sides of which are respectively provided with a transparent film layer and an insulating layer, wherein the transparent film layer fits with the sample carrier and is equipped with cell tissue samples, and a first electrode is disposed between the insulating layer and the sample carrier; a sample collector which is filled with a cell adhesion material for the adhesion of cell tissues and is also provided with a light-sensitive material for photoelectric reaction after laser irradiation; and a power supply device, the positive electrode of which forms break-make stations with the first electrode through a first switch. By the above scheme, the disadvantage that the cell tissue samples during the collection process are polluted and that requirements on materials of the sample carrier and the sample collector are high, carried static charges are inconvenient to preserve and the collection efficiency is poor in the prior art is avoided. According to the cell collection device and the method thereof, functions are stable, and cell tissue samples will not be damaged.

Description

technical field [0001] The invention relates to a collection device, method and system for collecting cells after laser microdissection. Background technique [0002] In life science research, it is necessary to conduct gene and protein research on a specific cell (group) in a tissue. The laser micro-cell dissection device or system is a high-end equipment developed to meet such needs, which can separate a specific or characteristic cell (group) from a variety of tissues, thereby avoiding the need for experimental Specimens are contaminated by other cells, bacteria, or other impurities, making the analysis of genes and proteins more accurate and specific. The core technology that distinguishes various laser micro-cell dissection systems is the different collection methods or devices for non-polluting collection of cut cells. [0003] U.S. Patents US 7807108B2, US 6907798B2, etc. disclose cell collection devices, which are placed directly below the sample, and the cells (cl...

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages. First: it can only be applied to polyimide films, and it is a polyimide film with negative charges in advance. Materials that meet this requirement are not easy to find in the market.

Second: Because it is difficult for the industry to produce polyimide films with a thickness of less than 2 microns, and thicker films are difficult to cut by laser or leave burnt marks on the edge of the cutting line after cutting

Third: Difficulty spreading negatively charged polyimide films on microscope slides

Fourth, the negatively charged polyimide film is prone to loss of charge during storage, which reduces the efficiency of cell collection

Fifth, it is difficult to find a positively charged collection material (agar is not charged). Even if it is found, the positively charged collection material is easy to lose as it is aged

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