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Method for breeding and separating high arsenic oxidizing bacteria

A high-arsenic oxidizing bacteria and separation method technology, which is applied in the field of leaching bacteria for efficiently oxidizing arsenic-containing sulfide ores, especially arsenic pyrite, can solve the problems of lack of arsenic-resistant bacteria, arsenic sand oxidation potential or weak oxidation potential, etc.

Inactive Publication Date: 2012-08-01
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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Problems solved by technology

This screening method is enriched by adding a certain amount of synthetic arsenic compounds and available energy sources or organic carbon sources to the artificial medium. Since the properties of the added arsenic compounds and energy substances are quite different from those of natural arsenopyrite, the resistance to enrichment and isolation Arsenic bacteria often do not have the potential to oxidize arsenopyrite or the oxidation potential is very weak, and the real arsenopyrite oxidizing bacteria are often eliminated

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  • Method for breeding and separating high arsenic oxidizing bacteria
  • Method for breeding and separating high arsenic oxidizing bacteria

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[0016] Collect tailings samples from the long-term tailings area of ​​high-arsenic gold mines and high-arsenic gold concentrate warehouse drainage ditch mud samples, mix the samples in equal amounts, add 2 times the volume of dilute sulfuric acid aqueous solution (pH2.0), slowly stir and mix, Make a suspension.

[0017] Take a sterilized 250mL Erlenmeyer flask, add 150mL medium 1, insert the above sample suspension according to the inoculum amount of 5%, adjust the pH to 2.0 with dilute sulfuric acid, shake and cultivate at 30°C, and regularly test the culture medium Fe 2+ , when Fe 2+ When the oxidation rate reaches over 95%, re-add FeSO to the culture medium according to the formula ratio 4 ·7H 2 O, and make up the culture solution to the original volume (150mL) with sterilized water, and continue shaking culture for 10 days, which is the first generation enrichment.

[0018] The first-generation enrichment was transferred to 150mL medium 2 at an inoculum amount of 2%, c...

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Abstract

The invention relates to a method for breeding and separating mineral leaching bacteria from an environmental sample, wherein the mineral leaching bacteria have high arsenic resistance and can efficiently oxidize arsenious sulfide ore, particularly arsenopyrite. The sample is made into turbid liquid, a culture medium 1 is inoculated in the turbid liquid, first-generation richment is obtained by means of shake culture at the temperature of 30 DEG C, a culture medium 2, a culture medium 3 and a culture medium 4 are sequentially added in the first-generation richment, three generations of richment are continuously cultured under the same conditions, and finally fourth-generation richment is obtained. The fourth-generation richment is continuously diluted and separated at the same temperature, and finally, dilution 4 is obtained, namely purified high arsenic oxidizing bacterial strains are obtained. Gold concentrate with containing 19.2% of arsenic is pre-oxidized, the arsenic content of oxidizing slag can be reduced by 93.02%, and the weight of the oxidizing slag can be reduced by 51.24%. Gold extraction experiments indicate that the gold leaching rate of the oxidizing slag reaches 95.78% and is five times that of the gold concentrate not pre-oxidized by the bacteria.

Description

Technical field: [0001] The invention relates to a method for breeding and isolating ore-leaching bacteria with strong arsenic resistance and high-efficiency oxidation of arsenic-containing sulfide ores, especially arsenopyrite, from environmental samples. Background technique: [0002] About 2 / 3 of the world's gold ore resources in my country and about 1 / 3 belong to arsenic-containing gold ore. Arsenic-containing gold ores are refractory gold ores, and gold is usually wrapped in arsenic-containing sulfide ores. If no pretreatment is carried out, the recovery rate of gold is very low. At present, the pretreatment technology of arsenic-containing gold ore commonly used at home and abroad is pre-oxidation method, mainly including oxidation roasting method, pressurized oxidation method, chemical oxidation method and bacterial oxidation method, etc. (Zheng Cunjiang. Research and application of arsenic-containing refractory gold ore [J]. Shaanxi Geology. 2003, 21(1): 88-97; Li J...

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Application Information

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IPC IPC(8): C12N1/20C22B3/18C12R1/01C22B11/00
CPCY02P10/20
Inventor 丁建南于一尊黄黄张国华计少石田晓娟张志红王东升黄江丽
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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