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microRNAs (micro ribonucleic acids) of mammary gland tissues of jinhua pig

A technology of mammary gland tissue and nucleotide sequence, applied in the field of Jinhua pig mammary gland tissue sequencing to analyze newly discovered microRNA sequences

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are relatively few miRNAs found in pigs. In the miRBASE database, there are 1424 pre-miRNAs (miRNA precursors) for humans, 720 for mice, 408 for rats, and only 228 for pigs.
miRNAs are species- and tissue-specific, so in recent years there have been more and more research reports on miRNAs in various tissues of pigs, such as skeletal muscle, heart, liver, thymus, fat, intestine, etc., but there are few miRNAs related to porcine mammary glands. research report

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  • microRNAs (micro ribonucleic acids) of mammary gland tissues of jinhua pig

Examples

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Embodiment 1

[0021] Example 1, Target Prediction of SEQ ID NO 90

[0022]The TargetScan software was used to predict the target gene of the sequence. Since the software does not contain pig information, the interaction between human mRNA and miRNA was used to predict the corresponding target gene. As a result, 3 targets were found, distributed in 3 The target genes are IQSEC1 (IQ motif and Sec7 domain 1), EPHB2 (EPH receptor B2) and SHANK3 (SH3 and multiple ankyrin repeat domains 3). After the KEGG search, it was found that IQSEC1 is a gene shared by humans and pigs, and the protein encoded by it is an ArfGEF family protein, which plays a role in the process of endocytosis; EPHB2 is also a gene shared by humans and pigs, encoding a 668-amino acid gene. Protein, belonging to the EPHB family, plays a role in axon guidance during organ development; SHANK3 exists in the human genome, but not found in pigs, and the protein encoded by it is involved in the change of glutamate receptors in the pr...

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Abstract

The invention discloses microRNAs (micro Ribonucleic Acids) in the mammary gland tissues of the Jinhua pig. The invention utilizes the sequencing technique to carry out deep sequencing on the mammary gland tissues of the Jinhua pig to obtain the sequences of mammary gland smallRNAs, and utilizes the bioinformatics method to analyze the sequences to obtain the variety and copy number of Jinhua pig miRNAs, so that certain predicted miRNAs in mammals, which are not included, are obtained, and the nucleotide sequences of the miRNAs are shown as SEQ ID NO:1 to SEQ ID NO:90. Certain miRNAs provided by the invention can be chosen for the research of a series of activities including porcine mammary gland lactation, lactation suppression and the like, and the microRNAs can help to increase the lactation volume of sows and the growth speed of piglets, and can also be used along with the currently discovered miRNAs in the customization of chips for the research of mammary gland activities or mammary gland diseases.

Description

technical field [0001] The invention belongs to the technical field of biology and agriculture, and in particular relates to a microRNA sequence newly discovered through sequence analysis of Jinhua pig mammary gland tissue. Background technique [0002] microRNA (miRNA) is a newly discovered endogenous single-stranded non-coding small RNA with a length of about 21-25 nucleotides. miRNA was first discovered in Caenorhabditis elegans in 1993, when Lee et al. the lin The -4 gene does not encode a protein, but it is a gene that can temporally regulate the late embryonic development of Caenorhabditis elegans. However the lin The discovery of -4 did not attract much attention until 2000, when Reinhart et al. discovered the second miRNA with similar function in C. elegans let -7 gene, gradually set off a miRNA research upsurge. The results of the study found that miRNA inhibits the translation of target mRNA or initiates the degradation of target mRNA through specific base pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113
Inventor 沈一飞张立凡王颖徐宁迎
Owner ZHEJIANG UNIV
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