Gelatin embolism microsphere and preparation method and application thereof

Abstract

The invention provides a gelatin embolism microsphere and a preparation method and an application thereof. The preparation method of the gelatin embolism microsphere comprises the following steps of: (1) preparing a gelatin aqueous solution of which the concentration is 10-50 percent g / ml; (2) adding the gelatin aqueous solution prepared in the step (1) into an emulsifier-containing oil phase of which the temperature is 50-60 DEG C, stirring for 10 minutes, lowering the temperature to 2-5 DEG C, stirring for 30 minutes at the stirring speed of 100-900 revolutions per minute, and standing to precipitate the microsphere; (3) pouring the oil phase out, repeatedly washing the oil phase left on the surface of the microsphere with acetone, and filtering the microsphere out; and (4) adding the microsphere obtained in the step (3) into a crosslinking agent aqueous solution of which the pH is 8-9 and the concentration is 1-15 percent g / ml, reacting at the temperature of 4 DEG C for 0.5-25 hours, and washing the microsphere with distilled water. The gelatin embolism microsphere provided by the invention is crosslinked uniformly, is regularly spherical, and is convenient for screening grading and accurate marking of the particle diameter; and the degradation time and elasticity of the gelatin embolism microsphere are more suitable for clinical application.

Description

technical field [0001] The invention belongs to the field of interventional medicine, and relates to a gelatin embolization microsphere for embolization therapy, a preparation method and application thereof. Background technique [0002] Interventional therapy is one of the fastest growing medical disciplines in the 21st century. At present, it has kept pace with medical treatment and surgical treatment, and has become the third treatment system. Embolization therapy is an important part of interventional therapy, and it is a minimally invasive treatment using modern high-tech means. Embolization therapy is a local treatment that artificially blocks blood vessels by introducing embolic agents into the human body through precision instruments such as special catheters and guide wires under the guidance of medical imaging equipment. Embolization therapy has achieved good results in the treatment of malignant tumors, uterine fibroids, hemangiomas, vascular malformations, and ...

AI Technical Summary

Problems solved by technology

However, the gelatin sponge particles prepared by many existing methods are difficult to accurately classify and mark their particle sizes due to their irregular shapes.

[0005] In addition, the gelatin sponge, gelatin particles, and gelatin microspheres prepared by the prior art have no definite degradation time, which leads to in vivo degradation and uncertain recanalization time, which is not conducive to clinical monitoring

The in vivo degradation time reported in some literatures is 14 to 90 days, which cannot meet the needs of all embolization applications.

[0006] The existing gelatin microsphere preparation technology does not pay attention to the elasticity of the microspheres. Microspheres with poor elasticity cannot pass through the embolization catheter smoothly, often causing catheter blockage and reflux.

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