Screen-printed electrode and multiple modification method thereof and method for detecting zearalenone

A technology of screen printing electrodes and zearalenone, which is applied in the field of detection, can solve the problems of unsuitable on-site detection, limited sensitivity, unfavorable routine detection, etc., and achieve the effects of easy promotion and application, high sensitivity and high accuracy

Active Publication Date: 2013-11-20
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

In addition, this method requires strict pretreatment of the test samples, high-performance liquid chromatography and other expensive instruments, and requires professional operators, which is not conducive to the use of on-site routine testing.
Although the ELISA method is simpler than HPLC and can be detected in large quantities, its sensitivity is limited and it is not suitable for on-site detection

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  • Screen-printed electrode and multiple modification method thereof and method for detecting zearalenone

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Embodiment

[0035] The schematic diagram of the embodiment of the present invention is as figure 1 As shown, the screen printing electrode includes a reference electrode 1, a working electrode 2, and a counter electrode 3; the working area of ​​the working electrode 2 is coated with Nafion-multi-walled carbon nanotubes 5-colloidal gold 4-chitosan Composite film layer; when using the screen printing electrode to detect ZEN in the sample, first drop OVA-ZEN6 on the working electrode that has been coated with the mixture film layer, and then add anti-ZEN monoclonal antibody 7 and The mixed solution of the sample extract to be tested is dried and then added with HRP-labeled goat anti-mouse secondary antibody 8, and finally added with the substrate solution H 2 o 2 and HQ.

[0036] The specific steps of the method for preparing the screen printing electrode of the present invention and detecting zearalenone with it are as follows:

[0037] Step 1, activation of carbon nanotubes

[0038] Di...

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Abstract

The invention discloses a screen-printed electrode and a producing method thereof and a method for detecting zearalenone. A working electrode area of the screen-printed electrode is coated with a mixture film of Nafion-multi-walled carbon nanotubes-colloidal gold-chitosan. The producing method includes the steps of mixing activated multi-walled carbon nanotubes with Nafion; preparing colloidal gold and uniformly mixing the multi-walled carbon nanotubes and the colloidal gold by using chitosan; coating the working area of the working electrode with the mixture; and drying at 37 DEG C to form a film. The method for detecting zearalenone (ZEN) comprises the steps of enabling ZEN toxin to be competitively bound with anti-ZEN antibodies if the ZEN toxin exists in the samples to be tested, so that the quantity of antibodies to be bound with ZEN-OVA on the electrode is reduced, horseradish peroxidase (HRP)-goat anti-mouse secondary antibodies on the electrode is also reduced, and delta-I is affected; and judging the content of ZEN in grain detection samples through a standard curve drawn according to standard ZEN. The method for detecting zearalenone is sole in detection object, high in accuracy rate and high in sensitivity.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a screen-printed electrode modified with a self-assembled film (Nafion film, carbon nanotube and colloidal gold), a preparation method thereof, and a method for detecting zearalenone. Background technique [0002] Zearalenone (ZEN) is a secondary metabolite produced by some strains of Fusarium under certain humidity and temperature conditions. Researchers have detected zearalenone in grains such as wheat, barley, corn, rye, and sorghum, and also in some animal tissues or products, including milk, eggs, etc. Zearalenone toxin can cause a variety of diseases in humans and animals, mainly carcinogenic and cancer-promoting toxicity. Reproductive system diseases have an important relationship. Zearalenone toxin has the characteristics of widespread distribution, long residual time, difficult to handle, and enhanced toxicity with other toxins. After joining the WTO, the internatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/333G01N27/26
Inventor 严亚贤王元凯孙建和王斌秦易
Owner SHANGHAI JIAO TONG UNIV
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