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Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography

An immunochromatography and creatine kinase technology, applied in the field of bioengineering, can solve problems such as distinguishing the severity of acute myocardial infarction, and achieve the effects of fast detection speed, single detection object and strong pertinence

Inactive Publication Date: 2012-07-04
正元盛邦(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the clinical standard of 5ng / mL alone cannot directly and quickly distinguish the severity of acute myocardial infarction, so it is also necessary to develop semi-quantitative colloidal gold test paper that can detect CK-MB 5-10ng / mL, the gray area of ​​CK-MB diagnosis. very necessary
At present, there are no relevant test strips for sale at home and abroad

Method used

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  • Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography
  • Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography
  • Method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0048] 1. Prepare CK-MB monoclonal antibodies 1 and 2. And clean the glassware needed for the experiment.

[0049] 2. Preparation of colloidal gold

[0050] First heat the 0.02% chloroauric acid solution to boil, then quickly add 2% trisodium citrate 2mL;

[0051] The color of the solution changed from light blue to dark blue. Continue heating to turn into wine red, continue to boil for 10 minutes; turn to transparent wine red, stop heating and continue to stir to room temperature. In this way, a 20 nm colloidal gold solution was prepared. Then use an electron microscope to check to ensure that the prepared gold particles are as consistent and uniform in size as possible, and the particle diameter is about 20nm, otherwise it will be remade.

[0052] 3. Preparation of colloidal gold markers

[0053] Take two 1.5mL test tubes, add 1mL colloidal gold solution respectively; add appropriate amount of borax buffer solution to adjust the pH to 8.0; add 20μg / mL CK-MB monoclonal an...

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Abstract

The invention discloses a detection method in the technical field of bioengineering, and particularly relates to a method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicating line immuno-chromatography. Acute myocardial infraction (AMI) is a very severe type of coronary heart diseases, is a common acute disease in middle-aged and aged people, and is high in mortality; statistics shows that about 1 / 3-1 / 2 patients are dead before they are sent to a hospital. In the future 20 years, acute myocardial infraction is the first reason causing patient death all around the world. CK-MB is the most reliable 'gold standard' for AMI detection by clinical doctors, and is high in specificity; currently, methods for detecting CK-MB commonly used clinically are enzyme-linked immunosorbent assay, radioimmunoassay, and the like, and the methods are time-consuming, labor-consuming, not in favor of popularization, and high in price. Immune colloidal gold technology develops rapidly recently, and is widely applicable to the detection of infectious diseases, early pregnancy, cancers and the like. The invention adopts colloidal gold immuno-chromatography, and establishes a method for rapid detection of CK-MB. And semi-quantitative detection of CK-MB in a gray area range of 5 ng / mL-10 ng / mL is realized.

Description

technical field [0001] The invention relates to a detection method in the technical field of bioengineering, in particular to a method for semi-quantitative diagnosis of creatine kinase isoenzyme by double indicator line immunochromatography. Background technique [0002] With the improvement of people's living standards in our country, the incidence of hyperlipidemia, hypertension and coronary heart disease has also increased significantly. Acute myocardial infarction (acute myocardial infarction, AMI) is a very serious type of coronary heart disease. It is coronary artery occlusion and blood flow interruption, which causes partial necrosis of myocardium due to severe and long-lasting ischemia, resulting in severe cardiac function. Damage, high mortality rate, according to statistics, about 1 / 3-1 / 2 of the patients died before being sent to the hospital. Acute myocardial infarction is more common in Europe and the United States, relatively less in my country, and slightly m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/573G01N33/558
Inventor 霍五奎岳晓燕马雪明刘寅王晶罗云萍张有青
Owner 正元盛邦(天津)生物科技有限公司
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