Composite nano material modified screen print electrode and method for detecting Fumonisin B1

Abstract

The invention discloses a composite nano material modified screen print electrode and a method for detecting Fumonisin B1. A working electrode of the screen print electrode is coated with a multi-walled carbon nano tube-colloid gold-chitosan mixture film. The preparation process includes the following steps; activating the multi-walled carbon nano tube; preparing the colloid gold; uniformly mixing the multi-walled carbon nano tube and the colloid gold through the chitosan; coating the mixture on the surface of the working electrode; and completing the preparation after the working electrode is dried at 37 DEG C and the film is formed. The method for detecting Fumonisin B1 provided by the invention is realized in the way that if FB1 exists in a to-be-detected sample, the FB1 competes with FB1 antibodies in the combination with FB1-OVA, so that the antibody amount combined with the FB1-OVA on the electrode is reduced, the amount of HRP-goat-anti-mouse second antibodies on the electrode is reduced, and the current variation value delta I is influenced; and then the content of FB1 in the to-be-detected sample of grains can be judged as per the standard curve drawn through an FB1 standard product. According to the invention, the detection object is single, the pertinence is strong, the accuracy is high, and the sensitivity is strong.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a screen-printed electrode modified with composite nanomaterials (carbon nanotubes and colloidal gold) and a method for detecting fumonisins B1. Background technique [0002] Fumonisin B1 (Fumonisin B1, FB1) is a secondary metabolite produced by Fusarium moniliforme under certain humidity and temperature conditions, and it is widely distributed all over the world. In 1988, Gelderblom et al first isolated fumonisins from the culture solution of Fusarium moniliforme. Fumonisins can contaminate corn and its products, and have been detected in some grain-based products such as noodles, beer, condiments, and even in asparagus. Fumonisins have been reported to be associated with equine leukomalacia, pulmonary edema syndrome in pigs, liver cancer in rats and other diseases. In addition to the above-mentioned diseases, studies in the Transkei region of South Africa and th...

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Problems solved by technology

In addition, this method requires strict pretreatment of the test samples, high-performance liquid chromatography and other expensive instruments, and requires professional operators, which is not conducive to the use of on-site routine testing.

Although the ELISA method is simpler than HPLC and can be detected in large quantities, its sensitivity is limited and it is not suitable for on-site detection

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