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Rapid test kit for detecting nitrite content in food and application thereof

A detection kit and nitrite technology, applied in the field of chemical detection, can solve the problems of expensive instruments and inability to adapt to real-time detection, and achieve the effects of low detection cost, easy popularization, on-site determination, and convenient storage

Inactive Publication Date: 2012-07-11
BEIJING PRIMEBIOTEK COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are also reports on the detection of nitrite by methods such as chromatography and automatic analyzers, these methods require expensive instruments and require professional and technical personnel to operate, which cannot meet the needs of real-time detection in actual production.

Method used

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  • Rapid test kit for detecting nitrite content in food and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A sample of a well-known brand of ham sausage purchased from a supermarket. Weigh 1.00 g of the uniformly crushed sample into a 50 mL plastic bottle, add distilled water to the 10 mL mark, stir evenly, and let stand for 5 minutes. Filter, take 1mL sample treatment solution in the chromogenic tube, shake until all the reagents in the chromogenic tube are dissolved, after 5 minutes, compare the color of the chromogenic tube solution with the standard color card, the measured nitrite content in the sample is 10mg / kg, for qualified samples.

Embodiment 2

[0036] Purchasing cabbage, spinach, cucumber, and rapeseed from the farmer’s market, crushed samples and stirred evenly, put 2.50g of the crushed samples into a 50mL plastic bottle, added distilled water to the 10mL mark, stirred evenly, and let stand for 5 minutes. Filter, take 1mL of each sample filtrate into a chromogenic tube, shake to dissolve the solvent, after 5 minutes, compare the color of the chromogenic tube solution with the standard color card, calculate the content of nitrite in each sample, and compare with For comparison with national standard methods, see the table.

[0037] Table 1 Comparison between the color card of the experimental sample and the national standard method

[0038]

Embodiment 3

[0040] For Cantonese-style bacon purchased from a supermarket, weigh 1.00 g of a uniformly crushed sample into a 50 mL plastic bottle, add distilled water to the 10 mL mark, stir evenly, and let stand for 5 minutes. Filter, take 1mL sample treatment solution in the chromogenic tube, shake until all the reagents in the chromogenic tube are dissolved, after 5 minutes, compare the color of the chromogenic tube solution with the standard color card, the measured nitrite content in the sample is 25mg / kg, for samples exceeding the standard.

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Abstract

The invention relates to a rapid test kit for detecting the nitrite content in food, and an application thereof, and belongs to the technical field of chemical detection. According to the present situation that the current nitrite residue is serious, and an economical and applicable detection product is in urgent need, a kit provided with low detection cost and being simple to operate, convenient to carry and suitable for on-site rapid detection is developed. The rapid test kit comprises a kit body, a plastic suction pipe, a 50 ml plastic bottle, a developing pipe, a small medicine spoon and a standard color card for nitrite. The detection method has the following steps that, after samples are treated with a sample treating liquid, compared the sample after the treatment with the standard color card after color reaction, whether the nitrite content in the sample is out of limit is judged, and the detection limit is 0.1 mg / kg.

Description

technical field [0001] The invention relates to a fast, sensitive and convenient kit for detecting nitrite in food, which belongs to the technical field of chemical detection. Background technique [0002] With the improvement of living standards, people's requirements for food no longer only focus on the quantity of consumption, but pay more attention to quality. Nitrite is one of the substances with strong acute toxicity in food additives. When nitrite in food enters the human body, it can produce chronic toxic effects, because sodium nitrite can react with ammonia in food or stomach to generate carcinogens Ammonium nitrite, a large amount of intake can make the hemoglobin in the blood become methemoglobin, lose the oxygen-carrying function, cause protracted cyanosis, damage the central nervous system due to hypoxia, dyspnea, circulatory failure, and can cause vasodilation , blood pressure drops. The main symptoms of poisoning are dizziness, headache, rapid heart rate, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N1/28
Inventor 时国庆白景妙
Owner BEIJING PRIMEBIOTEK COMPANY
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