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Simple bacillus product identification method

A technology of bacillus and identification method, which is applied in the direction of biochemical equipment and methods, microorganism measurement/inspection, etc., and can solve the restrictions on the use and promotion of bacillus products, difficult control of bacillus products, complicated detection methods of bacillus, etc. question

Inactive Publication Date: 2012-07-11
GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Bacillus detection methods are complicated, and have relatively high requirements on the culture medium and culture conditions. At present, most feed and breeding enterprises in my country still lack the necessary means for how to identify the activity of Bacillus. Relatively lagging behind, some fake and inferior bacillus products are difficult to control, which seriously restricts the use and promotion of bacillus products in animal feed and animal breeding
There is no report on a simple identification method for bacillus products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Bacillus products: commercially available Bacillus subtilis products;

[0017] 2. Method:

[0018] Activation of Bacillus subtilis-like products

[0019] Weigh 10g of Bacillus subtilis products, 30g of glucose, and 100g of soybean meal powder into 1.8kg of water at 33-37°C, and place for 4 hours.

[0020] Cultivation of Bacillus products

[0021] Put the activated bacillus products into a container containing 3kg soybean meal powder, mix evenly, cover with a piece of gauze, and place in a room at 25-30°C. Incubate for 48 hours.

[0022] Temperature records of the culture process of bacillus products

[0023] Measure the temperature of the fermentation material once in the morning, middle and evening.

[0024] Within 48 hours, the feed temperature rose to a maximum of 51°C.

[0025] 3. Quality determination of Bacillus subtilis products

[0026] The bacillus subtilis product is qualified and has bacillus activity.

Embodiment 2

[0028] 1. Bacillus products: commercially available Bacillus licheniformis products;

[0029] 2. Method:

[0030] Activation of Bacillus licheniformis products

[0031] Weigh 10g of Bacillus licheniformis products, 30g of glucose, and 100g of soybean meal powder into 1.8kg of water at 33-37°C, and place for 4 hours.

[0032] Cultivation of Bacillus licheniformis products

[0033] Put the activated bacillus products into a container containing 3kg soybean meal powder, mix evenly, cover with a piece of gauze, and place in a room at 25-30°C. Incubate for 48 hours.

[0034] Temperature records of culture process of Bacillus licheniformis products

[0035] Measure the temperature of the fermentation material once in the morning, middle and evening.

[0036] Within 48 hours, the feed temperature rose to a maximum of 47°C.

[0037] 3. Quality determination of Bacillus licheniformis products

[0038] The bacillus licheniformis product is qualified and has bacillus activity.

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Abstract

The invention relates to a simple bacillus product identification method, in particular to a simple method for identifying activities of bacillus licheniformis and bacillus subtilis (comprising bacillus natto) in a bacillus product. The method comprises the following steps of: (1) activating the bacillus product; (2) culturing the bacillus product; (3) recording the temperature in the culture process of the bacillus product; and (4) judging the quality of the bacillus product. The method is easy to operate and low in requirements for a culture medium and culture conditions, and can be supplied to feed and breeding enterprises which do not have microbiological examination means or do not have sound microbiological examination means at present and used for identifying the bacillus activity of the bacillus product and guiding purchase and use of the bacillus product.

Description

technical field [0001] The invention relates to a simple identification method for Bacillus licheniformis and Bacillus subtilis (including Bacillus natto) in bacillus products, specifically a method for identifying Bacillus licheniformis and Bacillus subtilis in bacillus products An easy method for the activity of Bacillus, including Bacillus natto. Background technique [0002] As beneficial bacteria, Bacillus licheniformis and Bacillus subtilis (including Bacillus natto) have been widely used in animal feed and animal breeding. They can strengthen the intestinal health of feeding animals, improve the function of animal immune system and the growth performance of animals played a very important role. However, the existing Bacillus detection methods are complicated, and have relatively high requirements on the culture medium and culture conditions. At present, most feed and breeding enterprises in my country still lack the necessary means for how to identify the activity of...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 潘军张伟强王瑞平薛德林
Owner GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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