Group of specific polypeptides and application thereof in preparing reagents for early diagnosis of lung cancer

A specific and versatile technology, applied in the field of preparation of early diagnostic reagents for lung cancer, to achieve huge market prospects and social and economic benefits

Inactive Publication Date: 2012-07-11
BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem of early diagnosis of lung cancer, the present invention discloses a group of specific polypeptides, the amino acid sequence of which is shown in sequence 1-29 in the sequence table, and the amino acid sequence and molecular weight M / Z of each polypeptide are shown in the following table

Method used

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  • Group of specific polypeptides and application thereof in preparing reagents for early diagnosis of lung cancer
  • Group of specific polypeptides and application thereof in preparing reagents for early diagnosis of lung cancer
  • Group of specific polypeptides and application thereof in preparing reagents for early diagnosis of lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Extraction of Serum Polypeptides from Metallic Copper Chelated Nano Magnetic Beads

[0015] 1. Materials:

[0016] Binding buffer solution: 0.1mol / l phosphate buffer solution, pH 4

[0017] Wash buffer solution: 0.1% formic acid in water

[0018] Eluent: 0.1% formic acid and 50% acetonitrile in water

[0019] Serum of 254 cases of lung cancer patients

[0020] 257 cases of control serum

[0021] Metal copper chelated nano magnetic beads: (self-made, patent application number: 200610002030.1)

[0022] 2. Method results:

[0023] (1) Preparation of metal copper chelated nano magnetic beads:

[0024] 1. Materials:

[0025] FeCl3: Analytical pure AR, batch number QD, March 11, 2004, produced by Sinopharm Chemical Reagent Co., Ltd.; PEG 1500: Mr1400-1600, batch number: 25300-68-3, FLUKA product; pyrrolidone (2-pyrrolidone): for synthesis, S34844146, product of MERCK-Schuchardt; diethyl ether: analytically pure, batch number: 20050315, produced by Tianjin Ch...

Embodiment 2

[0036] Example 2 Bioinformatics analysis of serum polypeptide map of lung cancer

[0037] 1. Software:

[0038] Biosun analysis software SVM package, http: / / www.csie.ntu.edu.tw / ~cjlin / libsvm

[0039] 2. Method

[0040] Sera from 254 cases of lung cancer and 257 cases of controls (healthy volunteers, pneumonia, chronic obstructive emphysema, COPD, benign lung tumors) were randomly selected to establish a lung cancer diagnostic model and screen for differential peptides. The cross validation and recognition capability of the obtained diagnostic model are both above 95%, and the remaining tumors and control cases are used for verification, and the diagnostic accuracy is above 90%.

Embodiment 3

[0041] Example 3 Sequence Identification of Lung Cancer Specific Serum Polypeptides

[0042] 1. Materials:

[0043] Serum from 10 cases of lung cancer patients; 10 cases of serum from healthy controls;

[0044] Magnetic beads (self-made, patent application number: 200610002030.1); 9.4 TQ-FT-ICR-MS (Bruker Company).

[0045] 2. Method results:

[0046] 1. Referring to Example 1, the serum peptides were extracted with magnetic beads, and the peptides extracted from 10 cases of lung cancer were mixed, and the peptides extracted from 10 cases of normal controls were mixed. The mixed peptide samples were concentrated in a centrifugal dryer to remove the organic phase. Prepare for mass spectrometry analysis to identify marker peptide sequences.

[0047] 2. The peptide sample was injected into nano LC-9.4T Q-FT-ICR-MS, gradient eluted, and the peptide was identified by tandem mass spectrometry, and the obtained spectrum was analyzed by DataAnalysis software (Bruker Company) to ob...

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Abstract

The invention discloses a group of specific polypeptides with amino acid sequences shown in SEQ ID Nos. 1-29 in the sequence list, and also discloses application of the specific polypeptides in preparing reagents for early diagnosis of lung cancer. The specific polypeptides of the invention are identified by the steps of performing comparative analysis through a serum polypeptide map in combination with bioinformatics software, identifying differentially expressing polypeptides and analyzing by mass spectrometry. The specific polypeptides disclosed by the invention can be used for preparing reagents for early diagnosis of lung cancer, and has a wide application prospect.

Description

technical field [0001] The present invention relates to a group of specific polypeptides, and also relates to the use of the group of polypeptides in preparing reagents for early diagnosis of lung cancer. Background technique [0002] Serum peptide group spectrum (referred to as peptide map) refers to the spectrum of accurate mass numbers of peptide (protein) groups in serum obtained through rapid analysis of mass spectrometry. The polypeptide (protein) peak identified by accurate mass number in the spectrogram can be further analyzed by biological mass spectrometry to obtain the protein sequence if necessary. The serum peptide map can be obtained by biological mass spectrometry analysis of trace blood in a few minutes, and it has shown good prospects in the early diagnosis of malignant tumors, which heralds an important breakthrough in the field of medical molecular diagnosis and brings new possibilities for the early detection of tumor patients. Gospel. Serum peptide pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K7/08G01N33/50
Inventor 张学敏王娜李爱玲何昆王杰
Owner BIOMEDICAL ANALYSIS CENT OF ACADEMY OF MILITARY MEDICAL SCI
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