Device and method for making liquid-based cytological smear in one step

Abstract

The invention discloses a device and a method for making a liquid-based cytological smear in one step. The device comprises a sample centrifuge, a hanging basket which is installed on the sample centrifuge and a smear making bottle which is reversely placed in the hanging basket. The smear making bottle comprises a bottle cap, a bottle body and a filter screen which is installed between the bottle cap and the bottle body. Slide loading holes for loading glass slides are symmetrically arranged on the left and right of the side wall of the bottle cap. The size of the holes is slightly larger than the size of the glass slides. The method comprises that the steps that after the smear making bottle which contains sample cells is shaken, the glass slides are loaded in the smear making bottle and the smear is made in one step by conducting centrifugal treatment to the smear making bottle. The device and the method for making the liquid-based cytological smear in one step have the advantages that the effect of combining a liquid storage bottle and a centrifugal smear making chamber into one is realized, the centrifugal smear making process is simplified, the transfer link in the smear making process is omitted, the cell analysis, the cell settlement and the smear output process are completed in one step, and the stability of smear making quality is improved. As a device and a method for making cell smears, the device and the method for making the liquid-based cytological smear in one step can be widely used in the field of cell smear making.

Description

technical field [0001] The invention relates to a film-making device and method, in particular to a liquid-based cytological smear one-step film-making device and method. Background technique [0002] In medicine, doctors can diagnose diseases such as cervical cancer in women by observing cell smears. Traditionally, cell slides are prepared manually, that is, the doctor manually spreads the sample evenly on a glass slide, fixes it with alcohol, stains it, and then observes it under a microscope. With the development of modern medicine, this traditional manual method for cell production has been phased out. [0003] At present, there are two main applications of cell sheeting technology: one is membrane-type thin-layer cell technology (TCT technology), which is to put the collected cells into a specimen bottle containing cell preservation solution, and use a stirrer to disperse and aggregate them in Cell particles together, and then use negative pressure to adsorb the cells...

AI Technical Summary

Problems solved by technology

Specifically, the disadvantages of TCT technology are that it is easy to lose some cells, the quality of film production is unstable, and it takes a long time and expensive consumables, etc.

The disadvantage of LCT technology is that the production steps need to be distributed, the steps are cumbersome, and too much equipment is required.

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