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Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon

A microbial agent and polycyclic aromatic hydrocarbon technology, which is applied in the direction of microorganisms, microbial-based methods, biological water/sewage treatment, etc., can solve the problems that the remediation effect of polycyclic aromatic hydrocarbon-contaminated soil has not been reported, and achieve good application prospects.

Inactive Publication Date: 2012-07-04
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mycobacterium is an important group of microorganisms with a strong ability to degrade PAHs with four or more rings. The screening of mycobacterial strains degrading PAHs has been reported, but the microbial agents of mycobacteria and their effects on PAH-contaminated soil The repair effect has not been reported

Method used

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  • Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon
  • Microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon

Examples

Experimental program
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experiment example 1

[0039] Experimental Example 1 The experiment of microbial bacterial agent of the present invention degrading polycyclic aromatic hydrocarbon-pyrene (Pyr)

[0040] According to the ratio of the number of live bacteria of Mycobacterium chrysogenum P6 and microacidophilic oligotrophomonas P56 as 1-2:1, a total of 0.5 ml of bacterial liquid of P6 and P56 was inserted into 20 mL of inorganic salt liquid medium (Na 2 HPO 4 .12H 2 O 8.8g, KH 2 PO 4 3.0g, NH 4 NO 3 1.0g, MgSO 4 .7H 2 O0.246g, FeCl 3 0.05g, CaCl 2 0.02g, trace element solution, 2.5ml, distilled water 1000ml. The composition of the trace element solution is: MnSO 4 .2H 2 O 0.06g, H 3 BO 3 0.03g, CoCl 2 .6H 2 O 0.04g, CuCl 2 .2H 2 O 0.01g, NiCl 2 .6H 2 O 0.02g, Na 2 MoO 4 .2H 2 O 0.03g, ZnCl 2 0.05g, pH7, distilled water 1000ml) serum bottle, add 5g / L pyrene acetone solution 0.1ml in advance in the serum bottle, and volatilize the acetone, the final concentration of fluoranthene in the culture medi...

experiment example 2

[0042] Experimental Example 2 The experiment of microbial bacterial agent of the present invention degrading polycyclic aromatic hydrocarbons-pyrene (Pyr) and fluoranthene (Flu) mixture

[0043] Change the concentration of polycyclic aromatic hydrocarbons in the serum bottle, add 0.1ml of acetone solution of 5g / L fluoranthene; at the same time, add 0.1ml of acetone solution of 5g / L pyrene, and volatilize the acetone, so that the medium contains pyrene at the same time and fluoranthene, their final concentrations were 100mg / L, respectively, and cultured on a shaker at 30°C in the dark after being inserted into the degrading strain, samples were taken on the 7th and 14th day, and the remaining polycyclic aromatic hydrocarbons in the culture medium were determined. The negative control CK is only added pyrene or fluoranthene to the inorganic salt medium without inoculation.

[0044] The result is as figure 2 As shown, the addition of fluoranthene did not affect the degradation ...

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Abstract

The invention provides a microorganism bacterium agent for degrading polycyclic aromatic hydrocarbon. The microorganism bacterium agent for degrading the polycyclic aromatic hydrocarbon consists of light yellow mycobacteria P6, and stenotrophomonas acidaminiphila P56. The bacterium agent has the capacity of degrading multiple kinds of polycyclic aromatic hydrocarbon of soil and sewage, an obvious effect of repairing soil polluted by the polycyclic aromatic hydrocarbon, and good application prospect; and the capacity of degrading polycyclic aromatic hydrocarbon (pyrene and fluoranthene), which are dissolved in a solution, by the microorganism bacterium agent is over 90 percent.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, in particular to a polycyclic aromatic hydrocarbon-degrading microbial bacterial agent containing mycobacterium light flavinum and microacidophilic stenotrophomonas. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) refer to hydrocarbon compounds composed of two or more benzene rings linked together, many of which have carcinogenic, teratogenic, and mutagenic effects, and their toxicity and carcinogenicity increase with the number of benzene rings And increase. PAHs in the environment mainly come from human activities, such as petroleum refining, transportation, and gas production. Due to the extensive use of petroleum and petroleum products, polycyclic aromatic hydrocarbons in the environment tend to increase and are widely distributed. The existence of various PAHs can be detected in the atmosphere, water, soil, sediment and food. [0003] The polycyclic aromatic ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/32C02F101/32
Inventor 宫小燕陆雅海
Owner CHINA AGRI UNIV
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