Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition

An extract and anti-tuberculosis technology, which is applied in the field of anti-tuberculosis catechu extract composition, can solve the problems of complex chemical components and achieve high effective content and improved therapeutic effect

Inactive Publication Date: 2015-04-29
许学志
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the literature has reported the method of obtaining monomeric compounds such as catechin and epicatechin from various plants including catechin through repeated chromatographic separation of normal phase and reversed phase silica gel, due to the chemical composition of catechin complex, but there are multiple phenolic hydroxyl groups in the structure of flavan compounds such as catechin and epicatechin, and the single chromatographic separation technology suitable for industrial production is used to enrich the high content, the total content is more than 50%, catechin and epicatechin Catechin is more difficult, so far no catechin and epicatechin catechin plant extracts or catechin and epicatechin monomer compounds or mixtures have anti-mycobacterium tuberculosis, especially anti-mycobacterium Literature reports on clinical drug-resistant Mycobacterium tuberculosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition
  • Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition
  • Catechu extract composition for resisting tubercle bacillus, preparation method of catechu extract composition, pharmaceutical preparation containing catechu extract composition, and application of catechu extract composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Extraction: 20g of traditional Chinese medicine catechu, crushed, soaked and extracted with 50% ethanol for 3 times, each time for 24 hours. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0032]2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, 75% ethanol through the treated HPD-100 macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of catechin and epicatechin derivatives were combined, concentrated under reduced pressure, and freeze-dried to obtain 9.9 g of extract. According to the standard method of "Pharmacopoeia of the People's Republic of China", the total content of main active ingredients including catechin and epicatechin is 53.2% after being measured by high performance liquid phase external standard method. Catechin and epicatechin were identified by using th...

Embodiment 2

[0034] 1. Extraction: 20g of traditional Chinese medicine catechin, crushed and added with 50% ethanol, soaked at 50°C for 3 times, 6 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0035] 2. Refining: the above-mentioned filtrate is eluted by gradient elution with water and 10%, 20%, 30%, 40%, 50%, and 75% ethanol through the treated HP-20 type macroporous adsorption resin, detected by HPLC, and collected rich in Fractions of catechin and epicatechin derivatives were combined, concentrated under reduced pressure, and freeze-dried to obtain 8.4 g of extract. According to the standard method of "Pharmacopoeia of the People's Republic of China", it is determined by the high-performance liquid phase external standard method, and the main active ingredients include catechin and epicatechin with a total content of 55.6%.

Embodiment 3

[0037] 1. Extraction: 20g of traditional Chinese medicine catechu, crushed, soaked in 50% ethanol overnight, heated and refluxed for 3 times, 3.0 hours each time. Combine the extracts, recover ethanol and concentrate to a thick extract, add distilled water to heat to dissolve, and filter to obtain a clear filtrate.

[0038] 2. Refining: the above-mentioned filtrate is washed with water, 10%, 20%, 30%, 40%, 50% and 75% concentration of methanol successively through the treated HP-20 macroporous adsorption resin, detected by HPLC, and collected rich Fractions containing catechin and epicatechin derivatives were combined, concentrated under reduced pressure, and freeze-dried to obtain 9.2 g of plant extract. According to the standard method of "Pharmacopoeia of the People's Republic of China", it is determined by the high-performance liquid phase external standard method, and the main active ingredients include catechin and epicatechin with a total content of 54.4%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a catechu extract composition for resisting tubercle bacillus, a preparation method of the catechu extract composition, a pharmaceutical preparation containing the catechu extract composition, and application of the catechu extract composition. The catechu extract composition contains a flavanoid compound of which the purity is more than 50%, and the flavanoid compound takes catechin and / or epicatechin as main components. The main structural formulas (I) and (II) of the flavanoid compound are shown in the specifications, wherein R1, R2, R3, R4 and R5 are respectively and independently selected from hydrogen, C1-C6 alkyl, beta-D-glucopyranesyl, SO3 or PO3. By adopting a chromatographic process for refining and purifying the catechu extract composition disclosed by the invention, the effective content is as high as 99.5%. The catechu extract composition and the pharmaceutical composition thereof have excellent effects on improving or treating pulmonary tuberculosis, treating ulcerative bone tuberculosis and AIDS (Acquired Immune Deficiency Syndrome) complicated by tuberculosis, treating drug resistant tuberculosis, and especially treating drug resistant tuberculosis and AIDS complicated by tuberculosis.

Description

technical field [0001] The invention relates to the technical field of natural medicines, in particular to an anti-tuberculosis catechu extract composition, a preparation method thereof, and pharmaceutical preparations and applications containing them. Background technique [0002] Catechu (catechu) is a dry extract concentrated from the dry branches of the plant Acacia catechu [Acacia catechu (L.) Willd.] in the leguminous family and decocted in water. It is recorded in "Drinking Zheng Yao", "Compendium of Materia Medica", "Materia Medica Seeking Truth", "Materia Medica Justice" and other ancient books, as well as historical editions of "Chinese Pharmacopoeia". Various sores, granulation promoting and pain relief, effects such as hemostasis and dampness absorption. It is used for the treatment of ulcers, eczema, aphtha, pain from falls, traumatic bleeding and other diseases. [0003] Modern research on catechins shows that catechins mainly contain catechin tannins, catech...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61K31/353A61P31/06
Inventor 许学志
Owner 许学志
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap