System and method for simultaneous detection of extracellular environmental toxicants and cell metabolism

Abstract

The invention discloses a system and method for simultaneous detection of extracellular environmental toxicants and cell metabolism. In the invention, a Zn<2+> chalcogenide glass sensitive membrane and a Cl<-> polyvinyl chloride sensitive membrane simultaneously deposit and combine on the surface of a light-addressable potentiometric sensor so as to form a Zn<2+> chalcogenide glass sensitive membrane-Cl<-> polyvinyl chloride sensitive membrane-light-addressable potentiometric sensor, which can quickly detect the Zn<2+> concentration of extracellular environmental toxicants and the Cl<-> ion concentration of cell metabolism at the same time, and infer the quantitative relationship between the two. A constructed micro-cavity can improve the sensitivity of ion concentration, and can ensure the consistency of detection conditions simultaneously, thus greatly reducing the part replacement cost and improving the service life of a sensor package unit. The above characteristics can meet the requirements for simultaneous detection of extracellular environmental toxicants and cellular metabolism, so that the system and method of the invention can be widely used in related fields of researchon heavy metal concentration and cell metabolism mechanism.

Description

technical field [0001] The invention relates to a detection technology, in particular to a detection system and a detection method for simultaneously detecting extracellular environmental poisons and cell metabolism. Background technique [0002] There are a wide variety of extracellular environmental toxicants, such as bacteria, viruses, toxic drugs, heavy metal poisons, etc., and sensors and detection methods for detecting these environmental toxicants have also been developed. At the same time, cell metabolism sensors and detection methods are also developing rapidly, such as patch clamp method, amperometric method, membrane capacitance method, etc. These technologies are also developing towards commercialization. However, there is no sensor and detection method that can simultaneously detect the concentration of heavy metal ions in the extracellular environment and the concentration of cellular metabolic ions. Therefore, it is impossible to quantitatively study the effec...

AI Technical Summary

Problems solved by technology

However, there is no sensor and detection method that can simultaneously detect the concentration of heavy metal ions in the extracellular environment and the concentration of cellular metabolic ions. Therefore, it is impossible to quantitatively study the effect of the concentration of extracellular heavy metal ions on cell metabolism, and it is impossible to give The quantitative relationship between the two has become a bottleneck hindering the rapid development of the analysis of extracellular environmental toxicants and cellular metabolic mechanisms

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