Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof
A technology of flower jujube honey and licorice polysaccharide is applied in the field of traditional Chinese medicine formula granule health food, which can solve the problems such as the blank in the research and development field of licorice polysaccharide.
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[0020] Preparation of licorice polysaccharide
[0021] (1) Obtaining of licorice aseptic seedlings: select licorice seeds full of particles and dark green, stir and sterilize the seeds on a magnetic stirrer with 10% sodium hypochlorite, the rotating speed is 300r / min, and the temperature is 25°C. Sterilization time was 30 minutes, MS culture contained 3% sucrose, pH 5.9 before sterilization, inoculated in a 50ml Erlenmeyer flask containing MS solid medium, placed in a light incubator at 25°C, and cultivated for 30 days to obtain licorice sterilized seedlings.
[0022] (2) Acquisition of licorice callus: the licorice callus was induced from the hypocotyls of the aseptic seedlings of licorice, and after several times of selection and subculture, the growth state was stable. The subculture medium of callus was MS medium, supplemented with 2,4-D (1 mg·L -1 ), NAA (1mg·L -1 ), 6-BA (0.2mg·L -1 ), 3% sucrose, pH 5.9 before sterilization, subculture cycle 28 days.
[0023] (3) Ob...
Embodiment 1
[0026] Extraction of licorice polysaccharide: take 500g licorice cell powder, add 20 times the weight of distilled water, let it stand for 12 hours, extract in a water bath at 100°C for 1 hour, repeat the extraction 3 times, combine the filtrates, concentrate to a certain volume, add 4 times the volume of absolute ethanol, Stand overnight at 4°C, remove the supernatant, remove protein by sevage method, and dry in vacuum to obtain licorice polysaccharide powder.
[0027] Preparation of honeysuckle extract: extract honeysuckle with water 3 times, add 10 times the weight of the medicinal material each time, extract for 1 hour each time, extract at 80°C, concentrate and dry the extract to form extract.
[0028] Preparation of jujube extract: Extract the jujube powder 3 times with water, the amount of each extraction is 15 times that of the raw medicinal material, the extraction time is 1.5 hours each time, the extraction temperature is 95°C, the extract is concentrated and dried to...
Embodiment 2
[0031] Extraction of licorice polysaccharide: Take 500g licorice cell powder, add 15 times the weight of distilled water, let it stand for 8 hours, extract in a water bath at 100°C for 2 hours, repeat the extraction twice, combine the filtrates, concentrate to a certain volume, add 4 times the volume of absolute ethanol, Stand overnight at 4°C, remove the supernatant, remove protein by sevage method, and dry in vacuum to obtain licorice polysaccharide powder.
[0032] Preparation of honeysuckle extract: extract honeysuckle with water once, adding water 15 times the weight of the medicinal material each time, extraction time is 3 hours each time, extraction temperature is 90°C, and the extract is concentrated and dried to form an extract.
[0033] Preparation of jujube extract: Extract the jujube powder with water twice, adding 10 times the amount of water each time as the raw medicinal material, extraction time is 2.5 hours each time, extraction temperature is 80°C, and the ext...
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