Dioscoreae Oppositae 'Qi' tissue culture seedling raising method
A technology of yam group and Qi yam, which can be applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems of difficulty in rooting, inability to rapidly multiply seedlings on a large scale, and low survival rate.
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[0049] The process flow of the seedling cultivation method of Qishan medicinal group is as follows:
[0050] 1. Preparation of explants
[0051] Take the potted Chinese yam cane and rinse it with tap water for 30 minutes, then transfer it to an ultra-clean workbench, disinfect it with 70% alcohol by volume for 5-10 seconds, then disinfect it with 2 wt% sodium hypochlorite for 20 minutes, and then rinse it with sterile water for 5-6 times. The canes were cut into 1-1.5 cm long segmented stem segments, placed on sterile filter paper to absorb excess water and then used for later use.
[0052] 2. Induction of callus
[0053] Inoculate the explants on MS+NAA 0.5~1.0mg / L+BA 0.1~0.2mg / L+sucrose 30g / L+agar 6.5g / L medium to induce callus, culture conditions: temperature 25~28℃, relative The humidity is 75-80%, the light is 16h / d, the light intensity is 3,000lux, and the pH value of the medium is 5.5-5.8.
[0054] 3. Adventitious shoots differentiated from callus
[0055] Select ca...
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