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Trichoderma hamatum biological agent for controlling soil borne disease for greenhouse vegetable cultivation

A technology for Trichoderma hacinata and soil-borne diseases, applied in the direction of biocides, chemicals for biological control, biocides, etc., can solve the problems of few reports in research, no patent application for Trichoderma hacinata, etc. Achieve the effect of improving soil micro-ecological environment, promoting growth and increasing production, and high microbial content

Active Publication Date: 2012-06-20
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the currently published patents, several Trichoderma species, preparation methods and their control effects on crop diseases are involved, but no patent application has been found for Trichoderma hamatum. Like Trichoderma harzianum, it is also a Trichoderma strain with biocontrol effects, but there are few reports on specific studies

Method used

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  • Trichoderma hamatum biological agent for controlling soil borne disease for greenhouse vegetable cultivation
  • Trichoderma hamatum biological agent for controlling soil borne disease for greenhouse vegetable cultivation
  • Trichoderma hamatum biological agent for controlling soil borne disease for greenhouse vegetable cultivation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment one: 1, a kind of trichoderma hooked biological preparation of prevention and treatment facility vegetable soil-borne disease, it is characterized in that: this biological preparation is prepared by following steps:

[0048] a. Preparation of expanded culture strains of Trichoderma hacinata: take the preserved strains of Trichoderma hacinata and insert them into PDA slant medium for activation, inoculate them on PDA plates and place them in a biochemical incubator at 28°C for 3-5 days , treat that it produces a large amount of conidia, promptly obtain Trichoderma hacinata expanded culture bacterial classification, described PDA culture medium is to be mixed by 200 grams of peeled potatoes, glucose 20 grams, agar powder 10 grams, add water to 1000ml;

[0049] b. Preparation of Trichoderma hamata seed fermented liquid: get 200 grams of peeled potatoes, 20 grams of glucose or sucrose, 1000 ml of water, adjust the pH value to 5-6, prepare the fermented liquid, an...

Embodiment 2

[0055] Embodiment two: a kind of trichoderma hooked biological preparation of prevention and treatment facility vegetable soil-borne disease, it is characterized in that: this biological preparation is prepared by following steps:

[0056] a. Preparation of expanded culture strains of Trichoderma hacinata: take the preserved strains of Trichoderma hacinata and insert them into PDA slant medium for activation, inoculate them on PDA plates and place them in a biochemical incubator at 28°C for 3-5 days , treat that it produces a large amount of conidia, promptly obtain Trichoderma hacinata expanded culture bacterial classification, described PDA culture medium is to be mixed by 200 grams of peeled potatoes, glucose 20 grams, agar powder 10 grams, add water to 1000ml;

[0057] b. Preparation of Trichoderma hamata seed fermented liquid: get 200 grams of peeled potatoes, 20 grams of glucose or sucrose, 1000 ml of water, adjust the pH value to 5-6, prepare the fermented liquid, and p...

Embodiment 3

[0063] Embodiment three: a kind of trichoderma hooked biological preparation of prevention and treatment facility vegetables soil-borne disease, it is characterized in that: this biological preparation is prepared by following steps:

[0064] a. Preparation of expanded culture strains of Trichoderma hacinata: take the preserved strains of Trichoderma hacinata and insert them into PDA slant medium for activation, inoculate them on PDA plates and place them in a biochemical incubator at 28°C for 3-5 days , treat that it produces a large amount of conidia, promptly obtain Trichoderma hacinata expanded culture bacterial classification, described PDA culture medium is to be mixed by 200 grams of peeled potatoes, glucose 20 grams, agar powder 10 grams, add water to 1000ml;

[0065] b. Preparation of Trichoderma hamata seed fermented liquid: get 200 grams of peeled potatoes, 20 grams of glucose or sucrose, 1000 ml of water, adjust the pH value to 5-6, prepare the fermented liquid, an...

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Abstract

The invention relates to a biological agent, in particular to a trichoderma hamatum biological agent for controlling soil borne disease for greenhouse vegetable cultivation. The biological agent is prepared through the following steps: preparing trichoderma hamatum enlarging culture; preparing trichoderma hamatum seed fermentation liquor; preparing solid fermentation culture medium; preparing trichoderma hamatum fermentations; smashing the trichoderma hamatum fermentations obtained through the step d and wheat bran respectively before use and sieving through a sieve of 60 to 80 meshes; mixingthe raw materials obtained through the step e with bergmeal, chitosan, potassium dihydrogen phosphate and biochemical fulvic acid, stirring uniformly and mixing completely to obtain jade-green powder, and determining that the content of conidiospore is 108 per gram, thereby obtaining the product. The product provided by the invention can not only effectively control soil borne disease for greenhouse vegetable cultivation but also can improve soil, is pollution-free and nuisanceless, can activate soil, has a wide range of application, and can improve the microenvironment of soil as well as induce and improve broad spectrum resistance of crops so as to promote growing of the crops.

Description

technical field [0001] The invention relates to a biological agent, in particular to a trichoderma hacinae biological agent for preventing and controlling soil-borne diseases of facility vegetables. Background technique [0002] The pathogenesis of soil-borne diseases of protected vegetables is complicated, the spread is fast, the damage is serious, and the yield loss is large, which often causes the deterioration of the soil ecological environment and can occur every year. Soil-borne diseases are also one of the main causes of continuous cropping obstacles. Crop joints provide a favorable place for the proliferation of pathogenic bacteria, which aggravates the occurrence of soil-borne diseases. There are many kinds of soil-borne diseases of facility vegetables, common ones are cruciferous sclerotinia, soft rot, damping-off, blight, blight, root rot, fusarium wilt and verticillium wilt, etc. Tomato, cucumber and sweet pepper bacterial wilt, root-knot nematode, etc. At pres...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N43/16A01N61/00A01P3/00A01P21/00C09K17/40C09K101/00
Inventor 康萍芝张丽荣杜玉宁沈瑞清孔令笛马建华白小军郭成瑾朱建祥吴惠玲刘娟
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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