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Detection method of mercury element

A detection method and element technology, applied in the fields of nanotechnology and analysis and detection, to achieve the effect of simple operation method, good sensitivity and selectivity

Inactive Publication Date: 2012-06-13
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, silver nanoparticles are usually used for colorimetric detection, but the present invention uses its quenching effect to design a detection method, and the work of analyzing and detecting the quenching characteristics of silver nanoparticles with DNA as a template is currently basically Did not appear, is a new field of research

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  • Detection method of mercury element
  • Detection method of mercury element
  • Detection method of mercury element

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 prepares silver nanoparticles with DNA as a template:

[0037] Prepare the solution required for the preparation of silver nanoparticles: dilute the DNA stock solution containing 23 bases with water to obtain a 5 μM / L solution, prepare a 1mM / L silver nitrate (AgNO3) solution, and prepare a 10mM / L sodium borohydride (NaBH 4 ) solution, prepare 10mM / L PBS buffer solution (pH=7.4) for use;

[0038] Add 490μL of PBS buffer solution (10mM / L) to a 1.5mL centrifuge tube; add 5μL of the prepared 5μM / L DNA solution and shake well; take 5μL of the prepared 1mM / L silver nitrate solution and add it, shake well , placed for 10 minutes (so that the silver ions can fully interact with the DNA); add 1 μL of 10 mM / L sodium borohydride solution for reduction, and shake the centrifuge tube vigorously. Due to the interaction between the silver ions and the bases on the DNA and then adsorbed to the DNA, silver nanoparticles with DNA as a template were formed, and 5 μL of 1mM / L me...

Embodiment 2

[0040] Embodiment 2 This detection method is to the selective fluorescence experiment of various metal ions:

[0041] Mix the DNA solution (5μM / L) and the silver nitrate solution (1mM / L) containing labeled fluorescein in a ratio of 1:1, shake well, and let it stand for 10 minutes; take PBS buffer solution (10mM / L, pH=7.4 ) 480 μL into the fluorescence pool with a volume of 1 mL; then add 10 μL of the above mixed solution and shake well; add 10 μL of different metal ion solutions (1 mM / L), so that the concentration of various metal ions is 20 μM, shake well, and place for 10 minutes; finally Add 2 μL of sodium borohydride solution (10mM / L) for reduction, shake and place for 20 minutes to allow the reaction to proceed fully. Test and record the fluorescence emission spectra to obtain the effects of adding different metal ions on the fluorescence of fluorescein.

[0042] Fluorescence spectrum analysis: from figure 2 It can be seen from the fluorescence spectrogram that when no ...

Embodiment 3

[0043] Embodiment 3 Various metal ions are to the influence experiment that the silver nanometer that takes DNA as template forms:

[0044] Mix fluorescein-labeled DNA solution (5 μM / L) containing 23 bases (5′-FAM-CCCCTAACCCTAACCCTAACCCC-3′) and silver nitrate solution (1 mM / L) at a ratio of 1:1 Leave it for 10 minutes; take 480 μL of PBS buffer solution (10mM / L, pH=7.4) and add it to an absorption pool with a volume of 1mL; then add 10 μL of the above mixed solution and shake well; add 10 μL of different metal ion solutions (1mM / L), Make the concentration of various metal ions 20μM, shake well, and let it stand for 10 minutes; finally add 2μL of sodium borohydride solution (10mM / L) for reduction, shake it and let it stand for 20 minutes, so that the reaction is fully carried out, test and record its absorption spectrum respectively, and get different Effect of addition of metal ions on the absorption spectrum of silver nanoparticles.

[0045] Absorption Spectrum Analysis: Fr...

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Abstract

The invention belongs to the field of nanotechnology and analysis and provides a detection method of mercury element based on interference of silver nanoparticle formation. The method comprises the following steps of: adding fluorescein (FAM) labeled DNA into PBS buffer, mixing by vortexing; adding a silver nitrate solution into the above solution, mixing by vortexing, standing still to make silver ions act fully with DNA and to be adsorbed onto DNA; adding sodium borohydride into the above solution, reducing silver ions on DNA to form silver nanoparticles and quenching the fluorescence of fluorescein. According to the method, mercury ions are infused into the solution to act for a period of time before adding sodium borohydride, and then the silver and mercury ions generate dissociative silver amalgam under the reduction of sodium borohydride so as to reserve the fluorescence of fluorescein. Therefore, the method can be used to detect mercury ions and also can be used to detect mercury vapor simultaneously. The method for the detection of mercury element (ions and vapor) has advantages of simple operation, economy and practicality, high specificity, high sensitivity, great innovation and application prospect in other targets.

Description

technical field [0001] The invention belongs to the field of nanometer technology and analysis and detection, and relates to a method for preparing silver nanoparticles with DNA as a template in nanomaterials and a method for detecting mercury elements using silver nanoparticles with DNA as a template. Background technique [0002] As a new nanomaterial, silver nanoparticles have become another research hotspot after gold nanoparticles. Similar to gold nanoparticles, silver nanoparticles have a characteristic plasmon resonance absorption peak between 390-420 nm. In addition, silver nanoparticles have a higher extinction coefficient, which makes it more sensitive when applied to colorimetric detection. Colorimetric detection methods based on silver nanoparticles have been applied to DNA, metal ions, proteins, etc. At the same time, silver nanoparticles have the property of Raman enhancement, and as Raman signal substances, they are widely used in the design of probes with Ra...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 杨荣华邓立李继山曹忠
Owner HUNAN UNIV
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