Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of chromosome suitable for karyotype analysis of rubus plants

A technology of karyotype analysis and Rubus genus, which is applied in the field of cytogenetics and cell biology, can solve the problem that the effect of chromosome samples is not as good as the technical process of the method, the method of enzymatic hydrolysis to remove the wall and low osmosis is cumbersome, and the cost of making chromosome karyotypes is increased, etc. problem, to achieve the effect of high specimen quality, easy coloring, and low production cost

Inactive Publication Date: 2012-06-13
SICHUAN AGRI UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0058] (2) The method of enzymatic hydrolysis to remove the wall and low osmosis method is loaded down with trivial details, and the technical requirement is higher, and reagent such as purchasing the cellulase and pectinase that needs a large amount is expensive at the same time, has improved the cost of making chromosomal karyotype greatly, and its The effect of Rubus plant chromosome specimens is not as good as the technical process of the method protected by this application. Figure 4 As shown, the materials used are R. parvifolius (2n=4x=28, D), yellow raspberry R.xanthocarpus (2n=2x=14, E), European red raspberry R.idaeus (2n=2x=14, F) and R. komarovii (2n=4x=28, G), - is 5um (Chen Ruiyang et al., 1993; Li Xiulan et al., 1993; Lin Shenghua, 1994)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of chromosome suitable for karyotype analysis of rubus plants
  • Preparation method of chromosome suitable for karyotype analysis of rubus plants
  • Preparation method of chromosome suitable for karyotype analysis of rubus plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0089] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0090] refer to figure 2 , a kind of chromosome preparation method suitable for Rubus plant karyotype analysis, its specific steps are:

[0091] 1) Material cultivation

[0092] Obtain adventitious roots through stem cutting propagation, take young shoots or hard branches with 3-5 nodes, cut them in a plastic greenhouse with vermiculite as the substrate, and carry out routine management. When the adventitious roots grow to 1-2cm, after cutting Take materials in 2-4 weeks, and take the growing root tips of strong cuttings;

[0093] 2) Pretreatment

[0094] Use 0.002mol L -1 8-Hydroxyquinoline was pretreated at 0-4°C for 24 hours;

[0095] 3) fixed

[0096] The root tip treated in step 2) was placed in Carnoy's fixative solution I (absolute ethanol: acetic acid = 3: 1, v / v), and fixed at 0-4 ° C for 24 h;

[0097] 4) save

[0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of chromosome suitable for karyotype analysis of rubus plants. The chromosome preparation method comprises the following specific steps of: material culturing; pretreatment; fixing; storage; dissolution; dyeing, smearing and tabletting; and microscopic examination and observation. The preparation method disclosed by the invention has the advantages of low cost, simple, convenient and speedy operation process and capability of obtaining an ideal chromosome specimen for karyotype analysis of the rubus plants.

Description

technical field [0001] The invention belongs to the field of cytogenetics and cell biology, and relates to a chromosome preparation method, in particular to a chromosome preparation method suitable for karyotype analysis of Rubus plants. Background technique [0002] Chromosome is the basis of genetic material in organisms and the main carrier of genetic material. Chromosome size, number, and shape (ie, karyotype characteristics) are relatively stable during the growth and generation of plants, and are not easily affected by changes in environmental conditions. They can largely reflect the characteristics of species and their genetic differences. Therefore, the karyotype characteristics and even the number of chromosomes of different species can provide cytological basis for the classification, phylogeny and genetic relationship identification of this genus. When conducting comparative studies on the number, shape, and karyotype of chromosomes in a specific group of cells, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/24
Inventor 王小蓉汤浩茹陈涛南红王燕刘泽静罗娅
Owner SICHUAN AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com