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Application of strain of high temperature and glucose resistant lactobacillus in lactate production

A high-temperature-resistant, high-sugar-resistant technology applied in the field of lactobacillus

Inactive Publication Date: 2012-06-13
TIANJIN POLYTECHNIC UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Deposit date December 08, 2010

Method used

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Embodiment Construction

[0019] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0020] Example 1:

[0021] The specific process is as follows:

[0022] 1. High temperature acclimatization

[0023] 1) Take a loop of Lactobacillus rhamnosus CGMCC No.1.2134 on the slant of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL of medium A (no agar), culture at 200rpm, 37°C for about 12h, Make the bacteria in the early logarithmic growth stage.

[0024] Medium A: (casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 20g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, sulfuric acid heptahydrate Magnesium 0.2g, manganese sulfate water 0.05g, calcium carbonate 20.0g, agar 15.0g, distilled water 1.0L, pH6.8).

[0025] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes...

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Abstract

The invention discloses application of a strain of high temperature and glucose resistant lactobacillus in lactate production, particularly relates to a high temperature and glucose resistant bacterial strain, and belongs to the field of lactobacillus. The lactobacillus is lactobacillus rhamnosus, the bacterial strain is filed in China General Microbiological Culture Collection Center with the filed number CGMCC No.4430, the bacterial strain CGMCC No.4430 can be resistant to the glucose of concentration reaching 270g / L which is increased by 95% as compared with that of original bacteria. After fermentation, the lactate content is 50g / L which is increased by 95% as compared with that of original bacteria. Various performance indexes of experiment tracks of a fermenting tank indicate that the bacteria strain is normal in metabolism, high in L-lactate production capability and low in heteroacid content and is a strain of excellent high temperature and glucose resistant lactobacillus rhamnosus.

Description

Technical field: [0001] The invention belongs to the field of lactobacillus, in particular to a strain of lactobacillus resistant to high temperature and high sugar. Background technique: [0002] In recent years, due to some special functions, the research of extremophiles has attracted more and more attention. Among them, high temperature resistant and hypertonic lactic acid bacteria are particularly prominent due to their wide application in various industries such as food, medicine and chemical industry. At present, its main applications include the following aspects: (1) Lactic acid: Lactic acid is an important food and chemical raw material, and can be used to prepare a degradable polymer material - polylactic acid, so it has attracted much attention in recent years. (2) Biological preservatives: Nisin is a biological preservative widely used in food and beverages in recent years. Compared with chemical preservatives, it is harmless to the human body and has a wider a...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12N1/20C12R1/225
Inventor 李政王玉张健飞李绩
Owner TIANJIN POLYTECHNIC UNIV
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