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High-permeability-resistant saccharomyces

A technology resistant to hypertonicity and yeast, applied in the field of yeast

Active Publication Date: 2013-06-05
NINGXIA RISINGMARK INTPROP CONSULTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Deposit date December 08, 2010

Method used

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Examples

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Embodiment Construction

[0020] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0021] Example 1:

[0022] The specific process is as follows:

[0023] 1. DES mutagenesis selection

[0024] 1) Take a ring of Saccharomyces cerevisiae CICC31481 on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask with 50mL of wort medium, and culture it at 200rpm at 30°C for about 10h, so that the bacteria are in the early logarithmic growth stage .

[0025] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minutes to collect the bacterial cells, and wash twice with normal saline.

[0026] 3) Dilute to 10 with pH7.0 phosphate buffer 7 individual / mL bacterial suspension.

[0027] 4) Take 32mL of pH7.0 potassium phosphate buffer, 8mL of bacterial suspension, and 0.4mL of DES in a 150mL Erlenmeyer flask pre-placed in the rotor and mix th...

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Abstract

The invention discloses high-permeability-resisting saccharomyces, particularly relating to a high-permeability-resistant brewing saccharomyces strain, belonging to the field of saccharomyces. The saccharomyces cerevisiae provided by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.4429; and each performanceindex obtained by the experiment of the fermentation tank shows that the saccharmyces strain has the advantages of normal metabolism, strong ethanol generating capacity and low heteroacid content, thereby being an excellent high-permeability-resisting saccharomyces strain.

Description

Technical field: [0001] The invention belongs to the field of yeast, in particular to hyperosmotic resistant Saccharomyces cerevisiae strains. Background technique: [0002] In recent years, due to some special functions, the research of extremophiles has attracted more and more attention. Among them, hypertonic yeast is particularly prominent due to its wide application in various industries such as energy, food, and medicine. Its main application comprises following several aspects at present: (1) ethanol: ethanol is a kind of important solvent and fuel, utilizes hyperosmotic yeast to be able to improve the concentration of substrate and product in the fermented liquid, has multiple advantages such as reducing cost; ( 2) Glycerol: When in a hyperosmotic environment, most types of yeast cells accumulate glycerol in the cell to facilitate the adjustment of osmotic pressure balance; (3) Erythritol: Erythritol is a moderate sweetness The bulking agent has a sweetness of 60% ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12N15/01C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 李政王玉张健飞
Owner NINGXIA RISINGMARK INTPROP CONSULTING CO LTD
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