Molecular breeding method based on polymorphism of candidate gene PRL (Prolactin)
A candidate gene and molecular breeding technology, applied in the field of chicken breeding, can solve the problems of low egg production and qualified egg rate, and achieve the effect of accelerating improvement
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[0016] Marker-assisted breeding was carried out according to the gene effect of the PRL gene mRNA sequence in the chicken gonadal axis on GenBank, that is, the hypothalamus-pituitary-ovary or testis tissue, on chicken reproductive traits, mRNA differential expression and translation protein differences.
[0017] A RT-PCR method for gene expression analysis was established.
[0018] The mutation of PRL gene in local chicken breeds was detected by sequencing typing method.
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