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Surface-bonded baicalin magnetic nano-particle, and preparation method and application thereof

A technology of magnetic nanoparticles and baicalin, which is applied in the field of phytochemistry, can solve the problems of inability to solve the problems of enrichment and separation of total flavonoids, and achieves the effect of avoiding toxic solvent residues

Active Publication Date: 2012-05-23
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This new separation material is used for column chromatography separation, which shows good selectivity and efficiency in the separation and purification of flavonoids, but it cannot solve the problem of rapid enrichment and separation of total flavonoids from complex systems of plant extracts

Method used

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  • Surface-bonded baicalin magnetic nano-particle, and preparation method and application thereof
  • Surface-bonded baicalin magnetic nano-particle, and preparation method and application thereof
  • Surface-bonded baicalin magnetic nano-particle, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Preparation of surface-bonded baicalin magnetic nanoparticles

[0031] Preparation of N-terminal Magnetic Nanoparticles: Fe 2+ and Fe 3+ Dissolve in deoxygenated high-purity water at a ratio of 1:2, add ammonia water dropwise to pH 10 under high-speed stirring, and react for 1.5 hours; after separation by magnet adsorption, add 1 times the amount of tetraethyl orthosilicate, and add dropwise under high-speed stirring Ammonia to pH 10, react for 2 hours; use magnet to separate and wash with pure water until neutral, then use ethanol as solvent, add 2 times the amount of 3-aminopropyltrimethoxysiloxane, stir at high speed for 10 hours, and use magnet to separate That is, amino-terminal magnetic nanoparticles are obtained.

[0032] Take 0.5g of amino-terminal magnetic nanoparticles and 0.1g of baicalin in a 250mL round-bottomed flask, disperse with 50mL of anhydrous dichloromethane, add 0.1g of dicyclohexylcarbodiimide, and use nitrogen to protect against water...

Embodiment 2

[0033] Example 2 Preparation of surface-bound baicalin magnetic nanoparticles

[0034] Take 0.1g of baicalin in a 250mL round-bottomed flask, add 15mL of thionyl chloride, reflux for 2 hours, steam off excess thionyl chloride under pressure, then add 50mL of anhydrous toluene to dissolve to obtain baicalin chloride, and pour it into the bottle under stirring. 0.5 g of amino-terminal magnetic nanoparticles were added to the mixture, nitrogen was protected against water vapor, and the mixture was refluxed for 12 hours. The obtained magnetic nanoparticles were adsorbed and separated by a magnet, redispersed and washed with methanol for three times, and dried under reduced pressure in a vacuum to obtain surface-bound baicalin magnetic nanoparticles.

Embodiment 3

[0035] Example 3 Application of surface-bonded baicalin magnetic nanoparticles

[0036] Weigh 0.2g of Chinese rose powder, add 500mL of water and ultrasonically extract for 45 minutes to obtain the water extract of Chinese rose (solution A); add 15 mg of surface-bound baicalin magnetic nanoparticles to solution A, shake vigorously for 5 minutes and then magnetically separate Pour off the supernatant, then add 1mL of methanol and shake vigorously for 1min, and magnetically separate the methanol solution (solution B) to enrich the flavonoids in rose flowers. The process is as follows: figure 2 shown.

[0037] Use electrospray mass spectrometry to detect solutions A and B respectively, as attached image 3 As shown, the results show that no flavonoid quasi-molecular ion peaks appear in solution A, and 7 flavonoids contained in rose flower can be detected in solution B enriched by surface-bound baicalin magnetic nanoparticles, The analysis of its cleavage fragments by electrosp...

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Abstract

The invention belongs to the technical field of phytochemistry and in particular discloses a surface-bonded baicalin magnetic nano-particle, and a preparation method and application thereof. The surface-bonded baicalin magnetic nano-particle disclosed by the invention is obtained by reacting baicalin and a magnetic nano-material. By utilization of the magnetic nano-material, flavones compounds inplant extract can be effectively enriched and can be conveniently and rapidly separated from a complex system of the plant extract in the presence of an external magnetic field. By utilization of theproperty of easily carrying out magnetic separation on the magnetic nano-particle, and the charge-transfer action and hydrogen bond action properties of baicalin molecules and aromatic ring substances, the prepared compound nano-material is used for enriching and separating the flavones component in the plant extract, thus, the surface-bonded baicalin magnetic nano-particle has a wide applicationprospect in the natural medicine industrial production.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and specifically discloses a surface-bonded magnetic nanoparticle of baicalin, a preparation method and an application thereof. technical background [0002] Flavonoids widely exist in the plant kingdom, especially in higher plants. Pharmacological studies have shown that they have good anti-oxidation, anti-cancer, anti-inflammatory, anti-proliferation and estrogen-like effects. At the same time, animal experiments and epidemiological studies have both It has been shown that the intake of foods high in flavonoids can effectively reduce the incidence of diseases such as cancer, cardiovascular disease, chronic inflammation and osteoporosis. [0003] Although liquid-liquid extraction has been widely used in industrial production as a conventional flavone pretreatment method (such as: acidifying the extract and extracting it with ethyl acetate), this method not only needs to consume a large am...

Claims

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Application Information

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IPC IPC(8): B01J20/22B01J20/30B01J20/28B01D15/08A61K36/00A61P9/00A61P29/00A61P35/00A61P39/06
Inventor 青琳森廖循薛莹熊静彭树林丁立生
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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