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Application of dehydroeffusol in preparation of drugs for tumor preventing and tumor angiogenesis inhibiting, health food or cosmetics

A technology of tumor angiogenesis and tumor angiogenesis, applied in anti-tumor drugs, cosmetic preparations, applications, etc., can solve the problem of small effect and achieve the effect of strong specific inhibitory effect

Inactive Publication Date: 2012-04-25
苏州中药研究所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, Chinese herbal medicine has not become the preferred anticancer drug at home and abroad, and the anticancer efficacy of existing clinical Chinese herbal medicine needs to be further improved.
For a long time, people have used traditional thinking, concepts and methods to develop anti-cancer Chinese herbal medicines. Although progress has been made, the results have been minimal

Method used

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  • Application of dehydroeffusol in preparation of drugs for tumor preventing and tumor angiogenesis inhibiting, health food or cosmetics
  • Application of dehydroeffusol in preparation of drugs for tumor preventing and tumor angiogenesis inhibiting, health food or cosmetics
  • Application of dehydroeffusol in preparation of drugs for tumor preventing and tumor angiogenesis inhibiting, health food or cosmetics

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1 dehydrofurfol

[0048] The dried rushes are crushed, extracted with ethanol, and the alcohol solution is concentrated under reduced pressure until there is no alcohol smell. The sample is loaded on a macroporous resin column, eluted with water and ethanol, and the eluted part of 85% ethanol is collected, and recovered to dryness under reduced pressure. Mix the sample with silica gel, load the sample on a silica gel column, detect and enrich the fraction by thin layer chromatography (TLC) to obtain the crude product of dehydroephedrine. The crude product of dehydrobutadiene is separated and purified repeatedly through silica gel column etc. to obtain the pure product of dehydrobutadiol (purity>90%), and then further purified by preparative HPLC, the purity of which reaches more than 95%. For the chemical structure and molecular formula of dehydroeufenol, see figure 1 .

Embodiment 2

[0049] Example 2 The effect of dehydrourban on the proliferation of various tumor cells / tumor stem cells:

[0050] 1. Name of tumor cell lines (62 strains)

[0051] Human megakaryoblastic leukemia cells MEG-01, Dami and Daudi, human chronic myelogenous leukemia cells PBL985 and K562, human myeloid leukemia cells HL-60, human leukemia cell line NB-4, human erythroid leukemia cells HEL, human Monocytic leukemia cells THP-1, UC-CUC, SHI-1 and SHI-2, human Burkitt's lymphoma cells Raji, Namalva, human T lymphocytic leukemia cells Jurkat, human T lymphocyte line H9, human acute lymphoma Cell leukemia T lymphocyte CCRF, human myeloma cell lines 8226, My-5, opm2, KMS11 and LP-1, human osteosarcoma cells Saos-2, MG-63, U-2OS and SW1353, human glioma stem cell line SU2 , human glioma cells SHG44, U251, A172 and U-87MG, human umbilical vein endothelial cells Eahy926, human umbilical vein endothelial cells ECV-304, human lung microvascular endothelial cells HLMVEC, human ovarian cancer ...

Embodiment 3

[0067] Example 3 Effects of Dehydroufen on Proliferation of Multiple Gastric Cancer Cells and Normal Gastric Epithelial Cells

[0068] 1. Cell lines: human gastric cancer cells SGC-7901, AGS and MGC803, human normal gastric mucosal epithelial cells GES-1.

[0069] 2. The drug under test: dehydroeufen, dissolved in dimethyl sulfoxide (DMSO), diluted to the desired concentration with RPMI 1640 or DMEM (high sugar) medium.

[0070] 3, experimental method: with embodiment 2

[0071] 4. Experimental results: dehydroeufenol showed a good proliferation inhibitory effect on a variety of gastric cancer cells, and with the increase of drug concentration, the inhibitory effect on the proliferation of various gastric cancer cells was correspondingly enhanced. The half-inhibitory concentrations of three kinds of gastric cancer cells SGC-7901, AGS and MGC803 were about 4.86, 8.47 and 4.17 μg / ml respectively; at the same time, dehydroeufen had only a relatively high concentration on normal ...

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Abstract

The present invention discloses an application of dehydroeffusol (the chemical name is 2,7-dihydroxy-1-methyl-5-vinylphenanthrene, the molecular formula is C17H14O2, and the molecular weight is 250) in preparation of drugs for tumor preventing and tumor angiogenesis inhibiting, or health food or cosmetics. According to the present invention, the results firstly show that: the Chinese herbal medicine monomer dehydroeffusol has activities of tumor inhibiting and tumor angiogenesis inhibiting, such that the prepared drug for tumor inhibiting and tumor angiogenesis inhibiting by the results can be used for treatments or preventions of diseases related to malignant tumors and tumor angiogenesis, and further can be used for treatments in the fields of tumor chemotherapy and / or adjuvant chemotherapy. According to the present invention, tumor stem cells, tumor cells and tumor angiogenesis are masterly adopted as the targets, and the medicinal efficacy and the action mechanism of the dehydroeffusol are studied and represented, such that the foundation is established for development of innovative anti-tumor traditional Chinese drug of the dehydroeffusol, and the scientific basis and the important information are provided for treatment of the malignant tumors by the traditional Chinese drug.

Description

technical field [0001] The invention relates to the application of dehydroephedrine in the preparation of antitumor drugs and angiogenesis inhibitors, health food or cosmetics. Background technique [0002] According to statistics from the Ministry of Health in February 2010, the number of cancer deaths worldwide reaches 10 million each year. At present, one-quarter of the global population's death is caused by cancer, and it is predicted that if the current trend continues, one-half of the deaths will be due to cancer by 2050. In my country, 2.2 million people suffer from cancer every year, and what is even more striking is that the incidence of malignant tumors is increasing at a rate of 2.5-5% per year, which has become the culprit that seriously endangers the lives of our people. Although countries around the world have made great efforts in the treatment of tumors, some progress has been made in some tumors, but overall the existing anti-tumor drugs are not effective f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P35/00A61P35/02A61P35/04A61P27/06A61P9/10A61P27/02A61P17/06A61P29/00A61P19/02A61P17/08A61P17/10A23L1/29A61K8/34A23L33/00
Inventor 周泉生顾振纶曹志飞朱峰妍蒋小岗成旭东
Owner 苏州中药研究所有限公司
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