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Rapid microcystin extracting and detecting method

A technology for microcystins and detection methods, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems that are not suitable for on-site real-time monitoring of sample processing and analysis, the need for large amounts of tested samples and reagents, sample extraction and pre-processing. Complex processing steps, etc., to achieve the effect of shortened retention time, simple instrument and simple operation

Inactive Publication Date: 2012-03-28
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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Problems solved by technology

These methods have their own advantages and disadvantages: the vacuum drying effect is better, but the equipment is expensive and difficult to popularize; the method of drying and heating will lead to the degradation of some heat-sensitive toxin variants; and the freeze-thaw method has low cell fragmentation rate and low extraction efficiency. lower disadvantage
In the experiment, it was found that the traditional extraction method has disadvantages such as long extraction time, heavy workload, complex sample extraction and pretreatment steps, and a large amount of tested samples and reagents. It is especially not suitable for on-site real-time monitoring and a large number of samples. processing and analysis

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Embodiment Construction

[0020] A method for rapid extraction and detection of microcystin, the specific steps are as follows:

[0021] A. Algae sample pretreatment 1: Algae microcystis samples in natural water bodies are collected with plankton nets with a pore size of 30-100 μm. After removing organic impurities such as leaves, duckweed, etc., it is repeatedly washed with distilled water for 2 or 3 or 4 times, and then concentrated into a viscous algae slurry with a plankton net with a pore size of 30-100 μm. Pay attention to drain the water as much as possible.

[0022] B. Homogenization treatment and dry weight determination 2: The algae slurry concentrated in step A above, after adding 2 times the volume of distilled water according to the volume ratio, homogenize with a homogenizer to make the algae cell clusters dispersed and the algae slurry density uniform. Take the algae slurry at 80°C for 24 hours, bake it to a constant weight, then cool it in a desiccator, and measure the dry weight on an anal...

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Abstract

The invention discloses a rapid microcystin extracting and detecting method comprising the following steps: firstly, conducting pretreatment on algae samples, removing impurities on microcystis flosaquae samples, cleaning the microcystis flosaquae repeatedly by distilled water, and filtering the microcystis flosaquae; secondly, conducting homogenate process and heavy weight measurement, adding algae slurry concentrated in the step one into the distilled water, stirring the slurry uniformly by a homogenizer, taking the algae slurry additionally for measuring the dry weight; thirdly, extracting toxin by methanol one-step method, calculating the measuring result of dry weight of samples in the step two, taking the algae slurry with known dry weight and volume, stirring, vibrating and mixing the algae slurry, then standing and extracting the algae slurry in a refrigerator; fourthly, conducting centrifugal purification, conducting centrifuge on extracted liquid processed in the step three, obtaining supernate, and obtaining clarified extraction liquid; fifthly, using a filter for filtering the supernate obtained from the step four; sixthly, conducting HPLC (High-performance liquid chromatography) detection, adopting optimized chromatograph condition for analyzing the toxin rapidly. The method has the advantages of high reliability, simple needed instrument, convenience in operation, high detection efficiency and the like, and is suitable for large-batch toxin extraction and analysis in a laboratory.

Description

Technical field [0001] The invention relates to the technical field of water quality detection, and more specifically to a method for rapid extraction and detection of microcystin in water bodies, and is particularly suitable for rapid sampling, extraction and detection of microcystin in water polluted by cyanobacteria blooms. The invention can be widely used in the on-site rapid detection of microcystins in water bodies. Background technique [0002] The frequency and intensity of domestic outbreaks of cyanobacterial blooms are increasing. As the monitoring of microcystin content in the water environment is listed as the safety standard for drinking water sources (WHO 1998), the routine detection of microcystin is increasingly causing countries around the world. Attention. Therefore, it is necessary to establish a high-efficiency extraction and detection technology of microcystin that can be promoted and applied in routine laboratories. [0003] In the extraction and detection o...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/14
Inventor 沈强胡菊香胡俊
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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