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Method for preparing crude paromomycin with membrane separation technology

A paromomycin and membrane separation technology, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of unshaped filter cake, high labor intensity, low filtration yield, etc., to avoid The effects of resin drift loss, reduction of secondary pollution, and low energy and material consumption

Inactive Publication Date: 2012-03-28
福建和泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The shortcomings of the current technology are: dynamic adsorption process, even if filter aid is added in the fermentation liquid plate and frame filtration process, the fermentation liquid is still difficult to filter, the filtration speed is slow, the filtration pressure is as high as 0.4-0.6Mpa, the filter cake is not formed, and the feed liquid In the static adsorption process, the ion exchange resin is separated from the residue in the fermentation broth, the loss of the ion exchange resin is serious, the yield is low, and the wastewater pollution is serious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Using 260L of fermentation broth containing paromomycin with a concentration of 3.5g / L as raw material, a ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 35L / h·㎡, the operating pressure is 2.5bar, and the operating temperature is 35°C Remove bacteria and residues from the fermentation broth under certain operating conditions. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30L / h·㎡, an operating pressure of 5.0bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane, and proteins and polysaccharides are removed. and colored substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 500. Under the operating conditions of the nanofiltration membrane flux of 30L / h·㎡, the operating pressure of 10.0bar, and...

Embodiment 2

[0022] Using 350L of fermentation broth containing paromomycin with a concentration of 3.3g / L as raw material, a ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 40L / h·㎡, the operating pressure is 3.0bar, and the operating temperature is 30°C Remove bacteria and residues from the fermentation broth under certain operating conditions. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30L / h·㎡, an operating pressure of 5.0bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane, and proteins and polysaccharides are removed. and colored substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 500. Under the operating conditions of the nanofiltration membrane flux of 30L / h·㎡, the operating pressure of 8.0bar, and ...

Embodiment 3

[0024] Using 350L of fermentation broth containing paromomycin with a concentration of 3.8g / L as raw material, a ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 40L / h·㎡, the operating pressure is 3.0bar, and the operating temperature is 30°C Remove bacteria and residues from the fermentation broth under certain operating conditions. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30L / h·㎡, an operating pressure of 5.0bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane, and proteins and polysaccharides are removed. and colored substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 500. Under the operating conditions of the nanofiltration membrane flux of 30L / h·㎡, the operating pressure of 8.0bar, and ...

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Abstract

The invention provides a method for preparing crude paromomycin with a membrane separation technology. The method comprises the following steps: 1, a material liquid containing paromomycin is processed by a microfiltration membrane, and remove the fermentation liquor thallus and the residue; 2, macromolecular impurities of proteins, polysaccharides and coloring substances in the microfiltered permeation liquid obtained in step 1 are removed; 3, the collected ultrafiltration permeation liquid is desalinated by a nanofiltration membrane at normal temperature and is enriched; and 4, the desalinated liquid is concentrated and dried to prepare the crude paromomycin. The method of the invention, which has the advantages of novel conception, mild production technology condition, environment protection, cleaning, simplicity, easy implement, short production period, low production cost, high extraction yield, and strong practicality, has a large popularization and application value.

Description

technical field [0001] The invention relates to a method for separating and producing aminoglycoside antibiotic paromomycin, in particular to a method for separating and producing paromomycin by membrane separation technology. Background technique [0002] Paromomycin is an aminoglycoside antibiotic, which is a specific drug for the treatment of amoebic dysentery, and can also be used for the treatment of bacillary dysentery and other gastrointestinal bacterial infections. It has inhibitory effect on amoebic protozoa and Shigella, and is mainly used for acute amoebic dysentery and bacillary dysentery; it is used orally to treat intestinal infections such as bacillary dysentery, amoebic dysentery and enteritis; by inhibiting intestinal symbiotic bacteria Cong metabolism, indirectly play the role of anti-amoeba, but also directly kill the amoeba trophozoites. [0003] Therefore, with the continuous development of the aquaculture industry, the development of an energy-saving a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/232C07H1/06
Inventor 陈灼湖杨斌
Owner 福建和泉生物科技有限公司
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