Method for simultaneously preparing albiflorin and paeoniflorin

A technology for paeoniflorin and paeoniflorin, which is applied in the field of medicine, can solve the problems such as disclosure of the process route of unhigh-purity paeoniflorin and high-purity paeoniflorin, harsh operating process control conditions, unsuitability for large-scale production, and the like. The operating process conditions are easy to control, the quality controllability is strong, and the energy consumption is low.

Inactive Publication Date: 2012-03-14
张作光
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method simulates moving bed chromatography to separate and prepare paeonifloride glycosides, adopts total glucosides of paeony as a raw material, and applies simulated moving bed chromatography to separate and prepare paeonifloride glycosides, the stationary phase of simulated moving bed chromatography adopts C18 silica gel, and the mobile phase A mixed solution of methanol or acetonitrile, water, formic acid, and isopropanol is used. In the mixed solution, by volume percentage: 10% to 50% of methanol or acetonitrile, 50% to 90% of water, 0 to 1% of formic acid, and isopropanol 0~2%, the sum of above-mentioned each component is 100%, although the purity of the paeonifloride prepared by this method can reach more than 90%, because this technology needs to use a large amount of expensive reverse phase silica gel (RP-C18 ), and the preparation process is complex, and the operating process control conditions are harsh, resulting in high product costs and not suitable for large-scale production
[0006] So far, there has not been a process route suitable for industrial production to simultaneously prepare high-purity paeonifloride and high-purity paeoniflorin

Method used

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  • Method for simultaneously preparing albiflorin and paeoniflorin
  • Method for simultaneously preparing albiflorin and paeoniflorin
  • Method for simultaneously preparing albiflorin and paeoniflorin

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1. Extraction of medicinal materials

[0035] 150 kilograms of dry peony roots, after being crushed into coarse powder, put into 2 tons of stainless steel traditional Chinese medicine extraction tanks, add mass percent concentration and be 70% ethanol solution, heat and reflux and extract three times, the volume of the ethanol solution used for extraction is 1200L successively, 900L, 900L, the extraction time is 3h, 2.5h, 2h in turn.

[0036] After combining the three extracts, use a vacuum decompression concentration tank (produced by Wuxi Huaxin Pharmaceutical Equipment Co., Ltd.) to carry out decompression concentration until it has no alcohol smell, and obtain a peony concentrate, wherein the temperature of decompression concentration is 70°C , the relative vacuum is -0.09~-0.075MPa, the relative density of the peony concentrate is 1.25, and the volume is 220L.

[0037] 2. Separation

[0038] 1) Add 700L of 95% ethanol to the peony concentrate, let it stand overni...

Embodiment 2

[0048] 1. Extraction of medicinal materials

[0049] 300 kilograms of fresh Paeoniae Alba root, chopped, put into 3 tons of traditional Chinese medicine extraction tanks, add ethanol with a concentration of 40% in mass percentage, heat and reflux for three times, the volume of ethanol used for extraction is 2200L, 1800L, 1800L, and extract The time is 3h, 3h, 2h in turn;

[0050] After the three extractions are combined, use a vacuum decompression concentration tank to carry out decompression concentration until there is no alcohol smell to obtain a peony concentrate, wherein the temperature of decompression concentration is 60°C, and the relative vacuum degree is -0.09~-0.08MPa , the relative density of peony concentrate is 1.21, and the volume is 250L.

[0051] 2. Separation

[0052] 1) Add 800L of 95% ethanol to the peony concentrate, let stand overnight, take the supernatant, concentrate to 250L, add 500L of water to dilute. Adopt macroporous adsorption resin column to ...

Embodiment 3

[0062] 1. Extraction of medicinal materials

[0063] 100 kg of dried peony roots were crushed into coarse powder, soaked in 400L of deionized water for 5 hours, put into a 1-ton stainless steel percolation tank, percolated with deionized water, and collected about 1500L of percolation liquid.

[0064] 2. Separation

[0065] 1) The percolation liquid is separated by a macroporous adsorption resin column. Among them, the macroporous adsorption resin is AB-8 type macroporous adsorption resin. The column volume of the macroporous adsorption resin column is 150L. For 50% ethanol elution, the elution flow rate is 100L / h, and the eluate is collected, and every 50L eluate is collected as a fraction;

[0066] 2) Use thin layer chromatography (TLC method) to detect the content of paeoniflorin and paeonifloride in the collected eluent, and the eluate fraction containing paeoniflorin or paeonifloride (or both) Merge together to obtain the resin column eluate, wherein, the thin-layer pl...

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Abstract

The present invention discloses a purification method for simultaneously preparing albiflorin and paeoniflorin. The method comprises: adopting a paeonia lactiflora medicinal material or a paeonia lactiflora extract as a raw material; adopting a column chromatography method to purify the paeoniflorin and the albiflorin. According to the present invention, the method of the present invention has characteristics of simple purification process, easy operation, high purification efficiency and short purification time; the high purity albiflorin and the high purity paeoniflorin can be prepared in the same process with low cost and low energy consumption, such that the purpose of environmental protection is achieved, economic benefits are improved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for separating and extracting compounds in the chemical field, in particular to a preparation process for plant natural physiologically active substances paeoniflorin and paeonifloride glycosides. Background technique [0002] Paeoniae Alba is a perennial herb of the Paeoniaceae Paeoniaceae Paeoniaceae family, and its dried roots are often used as medicine. Two species of peony were included in the 2010 edition of Chinese Pharmacopoeia. One is Radix Paeoniae Alba, which is the dry root of Paeonia lactiflora Pall. of the Ranunculaceae plant; the other is Radix Paeoniae Rubra, which is the dry root of Paeonia veitchii Lynch, a plant of the Ranunculaceae family. At present, there are many traditional Chinese medicine preparations containing peony on the market, such as Naoxueshuan Tablets, Yinao Fujian Capsules, Hemiplegia Recovery Tablets, Huisheng Zaizao Pills, Dahuoluo P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61K31/343A61K31/704A61K31/045A61P9/10
CPCA61K36/65A61P9/10C07H1/08C07H15/20
Inventor 顾正兵
Owner 张作光
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