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Preparation method of modified electrode for detecting uric acid and detection method

A technology for modifying electrodes and uric acid, which is applied in the field of electrochemical analysis and detection, and can solve problems such as the inability to accurately measure uric acid

Inactive Publication Date: 2012-02-15
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, because the electrochemical properties of ascorbic acid in the human body are similar to those of uric acid, the oxidation peaks of the two overlap on the bare electrode, and the purpose of accurate determination of uric acid cannot be achieved.

Method used

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  • Preparation method of modified electrode for detecting uric acid and detection method
  • Preparation method of modified electrode for detecting uric acid and detection method
  • Preparation method of modified electrode for detecting uric acid and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1, a kind of preparation method of the modified electrode that is used to detect uric acid, its steps are:

[0036] (1) Amino-terminated hyperbranched polyamide-amine HBP-NH 2 The preparation of: the raw material diethylenetriamine is put into the reaction vessel, start ice-water bath cooling, under N 2 Under the protection of the atmosphere, add a mixed solution of methyl acrylate and methanol dropwise to the reaction vessel, stop the ice-water bath, and react at room temperature for 4 hours to obtain a light yellow transparent liquid; , heated to 100°C, and continued to react for 2 h to obtain a viscous light yellow liquid. Add acetone to the viscous light yellow liquid, precipitate, filter, separate, and vacuum dry at 35°C to obtain a yellow viscous product HBP-NH 2 ; The volume ratio of raw materials diethylenetriamine, methyl acrylate and methanol is 1:0.4:1.4;

[0037] (2) HBP-NH 2 Preparation of modified carboxylated carbon nanotubes CCNT: Take CNT ...

Embodiment 2

[0041] Embodiment 2, a kind of preparation method of the modified electrode that is used to detect uric acid, its steps are:

[0042] (1) Amino-terminated hyperbranched polyamide-amine HBP-NH 2 The preparation of: the raw material diethylenetriamine is put into the reaction vessel, start ice-water bath cooling, under N 2 Under the protection of the atmosphere, add a mixed solution of methyl acrylate and methanol dropwise to the reaction vessel, stop the ice-water bath, and react at room temperature for 6 hours to obtain a light yellow transparent liquid; , heated to 180 °C, and continued to react for 6 h to obtain a viscous light yellow liquid. Add acetone to the viscous light yellow liquid, precipitate, filter, separate, and vacuum dry at 45 °C to obtain a yellow viscous product HBP-NH 2 ; The volume ratio of raw materials diethylenetriamine, methyl acrylate and methanol is 1: 0.6: 1.8;

[0043] (2) HBP-NH 2 Preparation of modified carboxylated carbon nanotubes CCNT: Take ...

Embodiment 3

[0047] Embodiment 3, a kind of preparation method of the modified electrode that is used to detect uric acid, its steps are:

[0048] (1) Amino-terminated hyperbranched polyamide-amine HBP-NH 2 The preparation of: the raw material diethylenetriamine is put into the reaction vessel, start ice-water bath cooling, under N 2 Under the protection of the atmosphere, add a mixed solution of methyl acrylate and methanol dropwise to the reaction vessel, stop the ice-water bath, and react at room temperature for 5 hours to obtain a light yellow transparent liquid; , heated to 130°C, and continued to react for 4 h to obtain a viscous light yellow liquid. Add acetone to the viscous light yellow liquid, precipitate, filter, separate, and dry in vacuum at 40°C to obtain a yellow viscous product HBP-NH 2 ; The volume ratio of raw materials diethylenetriamine, methyl acrylate and methanol is 1:0.5:1.6;

[0049] (2) HBP-NH 2 Preparation of modified carboxylated carbon nanotubes CCNT: Take t...

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Abstract

The invention discloses a preparation method of a modified electrode for detecting uric acid, characterized by firstly preparing amino hyper branched polyamide-amine HBP-NH2, then preparing HBP-NH2 modified carboxylated carbon nanotube CCNT-HBP-NH2, and then preparing a CCNT-HBP-NH2 / UCo3O4 / Nafion electrode modification liquid; and finally, coating the electrode modification liquid on the surfaceof the polished and cleaned glassy carbon electrode, and drying to obtain a composite film modified glassy carbon electrode. The prepared composite film modified glassy carbon electrode can be used for direct detection of uric acid concentration in urine and blood plasma, and has the advantages of sensitiveness, accuracy, stableness, high selectivity and the like.

Description

technical field [0001] The invention relates to a method for preparing a modified electrode, in particular to a method for preparing a modified electrode for detecting uric acid; the invention also relates to a method for electrochemically measuring uric acid with a modified electrode, which belongs to the technical field of electrochemical analysis and detection. Background technique [0002] Uric acid is the final product of the catabolism of purine nucleotides in the human body. Changes in the content of uric acid in body fluids can fully reflect the status of human metabolism, immunity and other functions, and can also indirectly reflect diseases related to purine metabolism such as gout, complete gene defect, hyperuricemia, etc. The uric acid content in the plasma of healthy people is 2.4×10 -5 ~5.2×10 -5 mol / L, the uric acid content in urine is 1.4×10 -3 ~4.4×10 -3 mol / L. [0003] There are many methods that can be used for the determination of uric acid, the ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 王明艳张东恩
Owner HUAIHAI INST OF TECH
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