Method for extracting ecdysterone from plant

A technology of ecdysterone and plants, applied in the field of extracting ecdysterone from plants by enzymatic hydrolysis and membrane filtration technology, can solve the problems of cumbersome process operation, high production cost, low extraction rate, etc., achieve simple operation and reduce damage , the effect of reducing the extraction temperature

Inactive Publication Date: 2012-02-15
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, the existing process has many technical defects such as high energy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Commelinaceae plant pearl dew grass is crushed, 10kg is added to the extraction tank, 30g of cellulase is dissolved in 10kgpH5 aqueous solution and added to the extraction tank, after enzymolysis at 50°C for 2 hours, the temperature is raised to 80°C, and then added 60kg heat preservation and soaking for 2 hours, filter the liquid and add 50kg water for heat preservation for 1 hour, filter the extract and add hollow cellulose ultrafiltration membrane with a molecular weight cut off of 2000 for ultrafiltration, and add the permeate to the hollow cellulose nanofiltration membrane with a molecular weight cut off of 300 to concentrate, To obtain a concentrated solution, add an equal volume of 95% ethanol solution to filter, then add an equal volume of ethyl acetate to extract once, add 50 g of activated carbon to the raffinate to reflux for decolorization, filter the decolorized solution to concentrate to alcohol-free crystals, filter the crystals and dry to obtain Ecdystero...

Embodiment 2

[0019] Pulverize the Lamiaceae plant Zijinpan, take 10kg and add it to the extraction tank, weigh 20g of amylase and dissolve it in a 10kgpH4 aqueous solution and add it to the extraction tank. After enzymolysis at 40°C for 3 hours, raise the temperature to 70°C, and then add 50kg Insulated and soaked for 2 hours, the filtered liquid was then incubated with 50 kg of water for 1 hour, the filtered extract was added to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 5000 for ultrafiltration, and the permeate was added to a hollow cellulose nanofiltration membrane with a molecular weight cut off of 200 to concentrate to obtain Concentrated solution, add an equal volume of 95% ethanol solution to filter, then add an equal volume of ethyl acetate to extract once, add 80g of activated carbon to the raffinate to reflux for decolorization, filter and concentrate the decolorized solution to alcohol-free crystals, filter the crystals and dry them to obtain m...

Embodiment 3

[0021] Amaranthaceae plant Achyranthes bidentata is crushed, 10kg is added to the extraction tank, 10g of pectinase is dissolved in 10kg of pH5 aqueous solution and added to the extraction tank, after enzymolysis at 50°C for 3 hours, the temperature is raised to 70°C, and 50kg of heat preservation is added for soaking After 1 hour, add 50 kg of water to the filtered liquid and keep it warm for 1 hour, add the filtered extract to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 3000 for ultrafiltration, and then add a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 300 to concentrate the permeate to obtain a concentrated solution , add an equal volume of 95% ethanol solution to filter, then add 1 / 2 volume of ethyl acetate to extract twice, add 60g of activated carbon to the raffinate to reflux for decolorization, filter and concentrate the decolorized solution to alcohol-free crystals, filter the crystals and dry them to o...

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PUM

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Abstract

The invention discloses a method for extracting ecdysterone from a plant, which is characterized by comprising the following steps of: a, grinding a raw material, adding biological enzyme, performing enzymolysis at the temperature of between 40 and 50 DEG C for 2 to 3 hours, adding water, raising the temperature to be between 70 and 80 DEG C, preserving heat, soaking for 1 to 2 hours, extracting for two to three times, and filtering to obtain extract; and b, adding an ultrafiltration membrane into the extract for ultrafiltration, adding a nanofiltration membrane into permeate for concentrating, adding ethanol solution with the same volume as concentrated solution into the concentrated solution for filtering, adding ethyl acetate for extracting, adding activated carbon into raffinate for reflux decolorization, filtering and concentrating decolorized solution until ethanol is not crystallized, and drying crystals to obtain the ecdysterone. The method is easy to operate, high in extraction efficiency, low in energy consumption and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting ecdysterone from plants, in particular to a method for extracting ecdysterone from plants by enzymolysis and membrane filtration technology. Background technique [0002] Ecdysterone is also called ecdysone. In the 1950s, scientists purified two active substances from silkworm chrysalis, which can induce insect molting, so they were called α-ecdysone and β-ecdysone. Many analogues of ecdysteroids have been isolated from more than 1,000 kinds of plants. Yunnan Province, China has obtained a large amount of ecdysterone from plants such as Dewweed, Jiangsu Province from Achyranthes, and Zhejiang Province from Zinnia. What role phytoecdysterones play in plants is unclear. From the perspective of the relationship between plants and insects, phytoecdysterone is a protective substance that can promote early molting of larvae and turn them into adults, preventing insect larvae from invading plants too much. I...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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