Method for extracting ecdysterone from plant
A technology of ecdysterone and plants, applied in the field of extracting ecdysterone from plants by enzymatic hydrolysis and membrane filtration technology, can solve the problems of cumbersome process operation, high production cost, low extraction rate, etc., achieve simple operation and reduce damage , the effect of reducing the extraction temperature
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Embodiment 1
[0017] Commelinaceae plant pearl dew grass is crushed, 10kg is added to the extraction tank, 30g of cellulase is dissolved in 10kgpH5 aqueous solution and added to the extraction tank, after enzymolysis at 50°C for 2 hours, the temperature is raised to 80°C, and then added 60kg heat preservation and soaking for 2 hours, filter the liquid and add 50kg water for heat preservation for 1 hour, filter the extract and add hollow cellulose ultrafiltration membrane with a molecular weight cut off of 2000 for ultrafiltration, and add the permeate to the hollow cellulose nanofiltration membrane with a molecular weight cut off of 300 to concentrate, To obtain a concentrated solution, add an equal volume of 95% ethanol solution to filter, then add an equal volume of ethyl acetate to extract once, add 50 g of activated carbon to the raffinate to reflux for decolorization, filter the decolorized solution to concentrate to alcohol-free crystals, filter the crystals and dry to obtain Ecdystero...
Embodiment 2
[0019] Pulverize the Lamiaceae plant Zijinpan, take 10kg and add it to the extraction tank, weigh 20g of amylase and dissolve it in a 10kgpH4 aqueous solution and add it to the extraction tank. After enzymolysis at 40°C for 3 hours, raise the temperature to 70°C, and then add 50kg Insulated and soaked for 2 hours, the filtered liquid was then incubated with 50 kg of water for 1 hour, the filtered extract was added to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 5000 for ultrafiltration, and the permeate was added to a hollow cellulose nanofiltration membrane with a molecular weight cut off of 200 to concentrate to obtain Concentrated solution, add an equal volume of 95% ethanol solution to filter, then add an equal volume of ethyl acetate to extract once, add 80g of activated carbon to the raffinate to reflux for decolorization, filter and concentrate the decolorized solution to alcohol-free crystals, filter the crystals and dry them to obtain m...
Embodiment 3
[0021] Amaranthaceae plant Achyranthes bidentata is crushed, 10kg is added to the extraction tank, 10g of pectinase is dissolved in 10kg of pH5 aqueous solution and added to the extraction tank, after enzymolysis at 50°C for 3 hours, the temperature is raised to 70°C, and 50kg of heat preservation is added for soaking After 1 hour, add 50 kg of water to the filtered liquid and keep it warm for 1 hour, add the filtered extract to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 3000 for ultrafiltration, and then add a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 300 to concentrate the permeate to obtain a concentrated solution , add an equal volume of 95% ethanol solution to filter, then add 1 / 2 volume of ethyl acetate to extract twice, add 60g of activated carbon to the raffinate to reflux for decolorization, filter and concentrate the decolorized solution to alcohol-free crystals, filter the crystals and dry them to o...
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