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Method for preparing novel biological microcapsule for biological fluidized bed

A biological fluidized bed, microcapsule technology, applied in chemical instruments and methods, sustainable biological treatment, biological water/sewage treatment, etc., can solve the problems affecting the application of microcapsule carriers, poor mass transfer performance, low stability, etc. , to achieve good mass transfer performance, good biocompatibility, and improve the treatment effect.

Active Publication Date: 2012-02-15
江苏科易达环保科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing embedding technology affects the application of microcapsule carriers because of its poor mass transfer performance and low stability.

Method used

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  • Method for preparing novel biological microcapsule for biological fluidized bed
  • Method for preparing novel biological microcapsule for biological fluidized bed

Examples

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Embodiment 1

[0017] A novel biological microcapsule carrier for a biological fluidized bed, characterized in that the microcapsule is composed of an inner core and an outer membrane, and the inner core is a liquid sodium alginate gel, which contains powdered activated carbon and Immobilized dominant bacteria, the inner core is coated with chitosan, the chitosan is coated with sodium alginate film, and the embedded bacteria in the inner core material are o-dichlorobenzene efficient degradation bacteria.

[0018] The microcapsule carrier of the present embodiment is prepared by the following methods and steps:

[0019] (1) Preparation of microbial mixed solution: add emulsifier Tween 80 and powdered activated carbon to sodium alginate solution and stir to obtain solution A, wherein the mass fraction of sodium alginate is 2.0%, the concentration of Tween 80 is 0.5%, powder Activated carbon 0.75%; add 10% to 20% (V / V) o-dichlorobenzene efficient degrading bacteria liquid to solution A in solut...

Embodiment 2

[0029] A novel biological microcapsule carrier for a biological fluidized bed, characterized in that the microcapsule is composed of an inner core and an outer membrane, and the inner core is a liquid sodium alginate gel, which contains powdered activated carbon and Immobilized dominant bacteria, the inner core is coated with chitosan, and the chitosan is coated with a sodium alginate film, and the embedded bacteria in the inner core material are highly efficient p-chlorophenol degrading bacteria.

[0030] The microcapsule carrier of the present embodiment is prepared by the following methods and steps:

[0031] (1) Preparation of microbial mixed solution: add emulsifier Tween 80 and powdered activated carbon to sodium alginate solution and stir to obtain solution A, wherein the mass fraction of sodium alginate is 2.0%, the concentration of Tween 80 is 0.5%, powder Activated carbon 0.75%; add 10% to 20% (V / V) p-chlorophenol high-efficiency degradation bacteria wet cells in sol...

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Abstract

The invention discloses a method for preparing a novel biological microcapsule for a biological fluidized bed. The microcapsule is prepared through treating sodium alginate, powdery active carbon, calcium chloride and chitosan as capsule materials, and a dominant degradation bacterium as a capsule core, preparing calcium alginate gel beads through carrying out ion exchange on sodium alginate and calcium chloride, coating the surface of the gel beads with chitosan, liquefying the gel beads in sodium citrate, and coating the gel beads with sodium alginate. The diameter of the prepared microcapsule is 3.0-4.0mm, the membrane thickness of the prepared microcapsule is 10-20mum, the density of the prepared microcapsule is 1.05-1.08g / mL, and the maximum interception molecular weight of the prepared microcapsule is about PEG 4000, so a micromolecular substance with the relative molecular weight of less than PEG 1500 can freely go through the capsule membrane. Acidic conditions and most divalent cations allow the microcapsule to be stable, and monovalent cations and most anions are bad for the stability of the microcapsule.

Description

1. Technical field [0001] The invention belongs to the technical field of microbial treatment of sewage, and in particular relates to a preparation method of a novel biological microcapsule applied to biological fluidized bed wastewater treatment. 2. Background technology [0002] Microencapsulation technology is a bead-shaped, elliptical or ellipsoidal microcapsule formed by encapsulating enzymes, coenzymes, proteins and other biological macromolecules or microorganisms, animal and plant cells in a hydrophilic semi-permeable membrane. Macromolecules and cells are blocked inside or outside the membrane, allowing the free entry of oxygen, nutrients and other small molecules needed by cells and the free discharge of metabolites in the capsule, so as to achieve the purpose of catalysis, culture or immune isolation. At present, biological microencapsulation technology has applications in various fields such as food and fermentation industry, chemical synthesis industry, and envi...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/10
CPCY02W10/10
Inventor 李朝霞丁成杨唐仪严金龙许琦吴俊峰
Owner 江苏科易达环保科技股份有限公司
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